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標題: | Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus | 作者: | Lin, J.W. 蔡慶修 Ding, M.P. Hsu, Y.H. Tsai, C.H. 徐堯煇 |
關鍵字: | viral-rna replication;tract-binding-protein;3' untranslated region;gene-block protein-1;potexvirus rna;poliovirus rna;genomic rna;strand rna;3'-untranslated region;functional-analysis | Project: | Nucleic Acids Research | 期刊/報告no:: | Nucleic Acids Research, Volume 35, Issue 2, Page(s) 424-432. | 摘要: | The tertiary structure in the 3'-untranslated region (3'-UTR) of Bamboo mosaic virus (BaMV) RNA is known to be involved in minus-strand RNA synthesis. Proteins found in the RNA-dependent RNA polymerase (RdRp) fraction of BaMV-infected leaves interact with the radio labeled 3'-UTR probe in electrophoretic mobility shift assays (EMSA). Results derived from the ultraviolet (UV) cross-linking competition assays suggested that two cellular factors, p43 and p51, interact specifically with the 3'-UTR of BaMV RNA. p43 and p51 associate with the poly(A) tail and the pseudoknot of the BaMV 3'-UTR, respectively. p51-containing extracts specifically down-regulated minus-strand RNA synthesis when added to in vitro RdRp assays. LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK). When the chloroplast PKG levels were knocked down in plants, using virus-induced gene silencing system, the accumulation level of BaMV coat protein was also reduced. |
URI: | http://hdl.handle.net/11455/50470 | ISSN: | 0305-1048 | DOI: | 10.1093/nar/gkl1061 |
Appears in Collections: | 生物科技學研究所 |
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