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標題: Chloroplast phosphoglycerate kinase, a gluconeogenetic enzyme, is required for efficient accumulation of Bamboo mosaic virus
作者: Lin, J.W.
Ding, M.P.
Hsu, Y.H.
Tsai, C.H.
關鍵字: viral-rna replication;tract-binding-protein;3' untranslated region;gene-block protein-1;potexvirus rna;poliovirus rna;genomic rna;strand rna;3'-untranslated region;functional-analysis
Project: Nucleic Acids Research
期刊/報告no:: Nucleic Acids Research, Volume 35, Issue 2, Page(s) 424-432.
The tertiary structure in the 3'-untranslated region (3'-UTR) of Bamboo mosaic virus (BaMV) RNA is known to be involved in minus-strand RNA synthesis. Proteins found in the RNA-dependent RNA polymerase (RdRp) fraction of BaMV-infected leaves interact with the radio labeled 3'-UTR probe in electrophoretic mobility shift assays (EMSA). Results derived from the ultraviolet (UV) cross-linking competition assays suggested that two cellular factors, p43 and p51, interact specifically with the 3'-UTR of BaMV RNA. p43 and p51 associate with the poly(A) tail and the pseudoknot of the BaMV 3'-UTR, respectively. p51-containing extracts specifically down-regulated minus-strand RNA synthesis when added to in vitro RdRp assays. LC/MS/MS sequencing indicates that p43 is a chloroplast phosphoglycerate kinase (PGK). When the chloroplast PKG levels were knocked down in plants, using virus-induced gene silencing system, the accumulation level of BaMV coat protein was also reduced.
ISSN: 0305-1048
DOI: 10.1093/nar/gkl1061
Appears in Collections:生物科技學研究所

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