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|標題:||Structural and mutational analyses of cis-acting sequences in the 5 '-untranslated region of satellite RNA of bamboo mosaic potexvirus||作者:||Annamalai, P.
|關鍵字:||Bamboo mosaic virus;potexvirus;Satellite RNA;5 '-untranslated region;cis-acting sequence;RNA repfication;enzymatic structural probing;turnip crinkle virus;nucleotide-sequence;strand synthesis;replication;elements;localization;accumulation;nepovirus;necrosis;plants||Project:||Virology||期刊/報告no：:||Virology, Volume 311, Issue 1, Page(s) 229-239.||摘要:||
The satellite RNA of Bamboo mosaic virus (satBaMV) contains on open reading frame for a 20-kDa protein that is flanked by a 5'-untranslated region (UTR) of 159 nucleotides (nt) and a 3'-UTR of 129 nt. A secondary structure was predicted for the 5'-UTR of satBaMV RNA, which folds into a large stem-loop (LSL) and a small stem-loop. Enzymatic probing confirmed the existence of LSL (nt 8-138) in the 5'-UTR. The essential cis-acting sequences in the 5'-UTR required for satBaMV RNA replication were determined by deletion and substitution mutagenesis. Their replication efficiencies were analyzed in Nicotiana benthamiana protoplasts and Chellopodium quinoa plants coinoculated with helper BaMV RNA. All deletion mutants abolished the replication of satBaMV RNA, whereas mutations introduced in most of the loop regions and stems showed either no replication or a decreased replication efficiency. Mutations that affected the positive-strand satBaMV RNA accumulation also affected the accumulation of negative-strand RNA; however, the accumulation of genomic and subgenomic RNAs of BaMV were not affected. Moreover, covariation analyses of natural satBaMV variants provide substantial evidence that the secondary structure in the 5'-UTR of satBaMV is necessary for efficient replication. (C) 2003 Elsevier Science (USA). All rights reserved.
|Appears in Collections:||生物科技學研究所|
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