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標題: Sequences at the 3 ' untranslated region of bamboo mosaic potexvirus RNA interact with the viral RNA-dependent RNA polymerase
作者: Huang, C.Y.
Huang, Y.L.
Meng, M.S.
Hsu, Y.H.
Tsai, C.H.
關鍵字: minus-strand synthesis;synthesis in-vitro;3'-untranslated region;3'-noncoding region;genomic rna;virus;replication;initiation;binding;identification
Project: Journal of Virology
期刊/報告no:: Journal of Virology, Volume 75, Issue 6, Page(s) 2818-2824.
The 3' untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) genomic RNA was found to fold into a series of stem-loop structures including a pseudoknot structure. These structures were demonstrated to be important for viral RNA replication and were believed to be recognized by the replicase (C.-P. Cheng and C.-H. Tsai, J. Mel. Biol. 288:555-565, 1999). Electrophoretic mobility shift and competition assays have now been used to demonstrate that the Escherichia coli-expressed RNA-dependent RNA polymerase domain (Delta 893) derived from BaMV open reading frame 1 could specifically bind to the 3' UTR of BaMV RNA. No competition was observed when bovine liver tRNAs or poly(I)(C) double-stranded homopolymers,were used as competitors, and the cucumber mosaic virus 3' UTR was a less efficient competitor. Competition analysis with different regions of the BaMV 3' UTR shelved that Delta 893 binds to at least two independent RNA binding sites, stem-loop D and the poly(A) tail. Footprinting analysis revealed that Delta 893 could protect the sequences at Loop D containing the potexviral conserved hexamer motif and part of the stem of domain D from chemical cleavage.
ISSN: 0022-538X
DOI: 10.1128/jvi.75.6.2818-2824.2001
Appears in Collections:生物科技學研究所

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