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標題: Crucial role of the 5 ' conserved structure of Bamboo mosaic virus satellite RNA in downregulation of helper viral RNA replication
作者: Hsu, Y.H.
Chen, H.C.
Cheng, J.
Annamali, P.
Lin, B.Y.
Wu, C.T.
Yeh, W.B.
Lin, N.S.
關鍵字: turnip crinkle virus;defective interfering rna;single nucleotide;change;open reading frame;triple-gene-block;5'-untranslated region;coat protein;differential interactions;symptom attenuation;secondary;structure
Project: Journal of Virology
期刊/報告no:: Journal of Virology, Volume 80, Issue 5, Page(s) 2566-2574.
Satellite RNA of Bamboo mosaic virus (satBaMV), a single-stranded mRNA type satellite encoding a protein of 20 kDa (P20), depends on the helper BaMV for replication and encapsidation. Two satBaMV isolates, BSF4 and BSL6, exhibit distinctly differential phenotypes in Nicotiana benthamiana plants when coinoculated with BaW RNA. BSL6 significantly reduces BaMV RNA replication and suppresses the BaMV-induced symptoms, whereas BSF4 does not. By studies with chimeric satBaMVs generated by exchanging the components between BSF4 and BSL6, the genetic determinants responsible for the downregulation of BaW replication and symptom expression were mapped at the 5' untranslated region (UTR) of BSL6. The 5' UTR of BSL6 alone is sufficient to diminish BaW RNA replication when the 5' UTR is inserted in cis into the BaMV expression vector or when coinoculation with mutants that block the synthesis of P20 protein takes place. Further, the 5' UTR of natural satBaMV isolates contains one hypervariable (HV) region which folds into a conserved apical hairpin stem-loop (AHSL) structure (W. B. Yeh, Y. H. Hsu, H. C. Chen, and N. S. Lin, Virology 330:105-115, 2004). Interchanges of AHSL segment of RV regions between BSF4 and BSL6 led to the ability of chimeric satBaMV to interfere with BaMV replication and symptom expression. The conserved secondary structure within the HV region is a potent determinant of the downregulation of helper virus replication.
ISSN: 0022-538X
DOI: 10.1128/jvi.80.5.2566-2574.2006
Appears in Collections:生物科技學研究所

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