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標題: Purification and characterization of an antifungal chitinase in jelly fig (Ficus awkeotsang) achenes
作者: Li, Y.C.
Chang, C.T.
Hsiao, E.S.L.
Hsu, J.S.F.
Huang, J.W.
Tzen, J.T.C.
關鍵字: achene;antifungal;chitinase;jelly fig;pericarp;acidic pectin methylesterase;ripening tomato fruits;expression;cloning;proteins
Project: Plant and Cell Physiology
期刊/報告no:: Plant and Cell Physiology, Volume 44, Issue 11, Page(s) 1162-1167.
A method was developed to purify a 30-kDa protein from jelly fig (Ficus awkeotsang) pericarp, including preparation of jelly curd from achenes, extraction of proteins from the curd, and isolation of the 30-kDa protein by anion-exchanger and gel filtration. Chitinase activity was detected in the purified 30-kDa protein by activity staining in both non-denaturing gel electrophoresis and SDS-PAGE. Isoelectrofocusing showed that the isoelectric point of the 30-kDa protein was lower than pH 3.5. The K-m, k(cat), optimal pH and temperature of this putative chitinase were determined to be 0.076 mM, 0.089 s(-1), pH 4, and 60degreesC, respectively. The purified 30-kDa protein was thermostable (retaining activity up to 65degreesC for several hours) and could be stored at 4degreesC for a year without apparent loss of chitinase activity. Antifungal activity of this putative chitinase was measured in terms of inhibition of Colletotrichum gloeosporioides spore germination.
ISSN: 0032-0781
DOI: 10.1093/pcp/pcg141
Appears in Collections:生物科技學研究所

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