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標題: Chemical modification of Nitzschia panduriformis’s frustules for protein and viral nanoparticle adsorption
作者: Wu, Meng-Chuan
Chang, Gary Ro-Lin
Suen, Shing-Yi
Lin, Chia-Feng
Chou, Hong-Nong
Lai, Su-Yuan
Wang, Min-Ying
關鍵字: Diatom;Immobilized metal affinity chromatography;Frustules;GFP;Virus-like particles
Project: Process Biochemistry, Volume 47, Page(s) 2204–2210.
The frustule of diatoms, through appropriate chemical modification, can be developed for a high adsorption
level of recombinant proteins and viral nanoparticles. Field emission scanning electron microscopy
(FE-SEM) analysis of clean frustules revealed a 3D loculate areolae structure (valvar phase porous pattern
of the siliceous cell wall). Isocyanatopropyl triethoxysilane (IPS) and iminodiacetic acid (IDA) were used
to immobilize Cu2+ ions (an average Cu2+ adsorption capacity about 190 mol of Cu2+/ml of the Cu2+-
coupled biosilica reached). FE-SEM, energy dispersion X-ray spectroscopy (EDS) and Fourier transform
infrared (FT-IR) were used to confirm the chemical modification of the Cu2+-coupled biosilica. Protein
adsorption was confirmed with the detection of a recombinant (His)6-tagged green fluorescent protein
binding using fluorescent microscopy. Infectious bursal disease virus VP2-441 subviral particles (SVPs)
were found to bind to the Cu2+-coupled biosilica (approximately 3
10−9 mol of VP2-441 SVPs/ml of
modified frustules), a level higher than the previously obtained 9
10−10 mol/ml for SVP binding using
a commercial Ni–NTA resin. These give diatom frustules the potential to be developed into a material
useful in viral nanoparticle purification systems or as a biosensor for the detection of viruses.
ISSN: 1359-5113
DOI: 10.1016/j.procbio.2012.08.015
Appears in Collections:生物科技學研究所

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