Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50976
標題: 九種非傳統食用植物水萃取物細胞毒性、致突變性及抗致突變性之研究
Evaluation of cytotoxicity, mutagenicity and antimutagenicity of nine non-traditionally edible plants
作者: 彭惠鉉
peng, Hui-Hsuan
關鍵字: non-traditionally edible plants;非傳統食用植物;mutagenicity;antimutagenicity;toxicity;fractionation;致突變性;抗致突變性;毒性;區分
出版社: 食品科學系
摘要: 
本研究是以安氏試驗法 (Ames test),分析九種新興蔬菜及野生可食用性
植物之毒性及致突變性,並對其抗致突變性加以探討,對於具有毒性的樣
品更進一步以彗星試驗法 (Comet assay)分析其細胞及基因毒性。由結果
顯示,八種非傳統性食用植物及過溝菜蕨在添加劑量分別為5 mg/plate
及0.1 ml/plate的範圍內對Salmonella typhimurium TA98及TA100皆不具
有致突變性。只有龍葵綠果在不添加S9的情況下對TA100及CHO細胞具有毒
性作用,且隨著添加劑量的增加而漸增。在添加劑量為1、3及5mg/plate
時,TA100的存活率分別為75、36及42%;就CHO細胞而言,添加劑量為0
、20、40、80、100、200、300、400mg/ml的情況下,其細胞存活率
為93.7、91.1、91.3、90.1、82.8、39.5、5.4及0.9%。在添加劑量
為0-80 mg/ml情況下,並不會誘導基因毒性作用。在抗致突變方面,樣品
對不同致突變劑的抗致突變效果為2-amino-3-methylimidazo(4,5-f)
quinoline (IQ)>Benzo[a]pyrene (B[a]P)>4-nitroquinoline-N-oxide
(NQNO)。對於IQ誘導TA98及TA100致突變系統而言,樣品之抗致突變效果
分別為:隼人瓜苗、龍葵>昭和草、黃麻嬰、落葵、過溝菜蕨>馬齒莧、
藤三七、雷公根;及隼人瓜苗、龍葵>昭和草、藤三七、黃麻嬰>馬齒莧
、雷公根、落葵、過溝菜蕨。對於B[a]P誘導TA98及TA100致突變系統而言
,樣品之抗致突變效果分別為:昭和草、隼人瓜苗>雷公根>馬齒莧、藤
三七、龍葵>黃麻嬰、落葵、過溝菜蕨(其中後三者不具有抗致突變效果
);及昭和草、龍葵>馬齒莧、黃麻嬰、過溝菜蕨>隼人瓜苗、雷公根>
藤三七、落葵(其中後二者不具有抗致突變效果)。對於NQNO誘導TA98及
TA100致突變系統而言,除了龍葵在TA100的系統具有很強的抗致突變效果
外,其餘樣品在TA98及TA100的系統中皆不具有抗致突變性;且在TA100的
系統中隼人瓜苗、馬齒莧、藤三七、雷公根、落葵及過溝菜蕨反而具有促
進致突變的效果。隼人瓜苗之水萃取物以100℃ 20min加熱處理後抗致突
變性有降低的趨勢,並發現樣品經打碎、離心、過濾及冷凍乾燥處理後可
產生熱穩定性的抗致突變物質。進而初步區分隼人瓜苗水萃取物之抗致突
變物,發現介於濾膜分子量30000以上的區分物,含有其主要的抗致突變
物質。分析組成物的特性,推測隼人瓜苗水萃取物之主要抗致突變物質可
能是過氧化酵素,而總多酚類化合物也可能是熱穩定性的抗致突變因子之
一。關鍵字:非傳統食用植物,致突變性,抗致突變性,毒性,區分

The Ames test was used to evaluate the toxic, mutagenic, and
antimutagenic effects from edible parts of nine non-
traditionally edible plants, including Crassocephalum
creidioides S. Moore (Cra), Sechium americanum Poir (Sec),
Portulaca oleracea L. (Por), Boussingaultia gracilis Miers var.
(Bou), Corchorus capsularis L. (Cor), Centella asiatica L. Urban
(Cen), Solanum nigrum L. (Sol), Basella rubra L. (Bas), and
Anisogonium esculentum Presl (Ani). Comet assay was further used
to evaluate the genotoxic effect of the plant extract with
cytotoxic effect. Only green fruit of Sol in the absence of S9
mix showed toxicity to TA100 and Chinese hamster ovary cell
(CHO) with dose-dependence. For TA100, viability were 75, 36 and
42% corresponding to the dose of 1, 3 and 5 mg/plate of green
fruit of Sol, respectively; For CHO cell, cell viability was
93.7, 91.1, 91.3, 90.1, 82.8, 39.5, 5.4 and 0.9% respect to the
dose of 0, 20, 40, 80, 100, 200, 300 and 400 mg/ml,
respectively. Also it did not induce DNA damge within the dose
range of 0-80 mg/ml.The antimutagenic potencies of the water
extracts of the same nine plants against the mutagenicity of
2-amino-3-methyl[4,5-f]quinine (IQ), benzo[a]pyrene (B[a]P), and
4-nitroquinoline-N-oxide (NQNO) to TA98 and TA100 were
investigated. For IQ in TA98, Sec and Sol exhibited strong
antimutagenic activity; Cra, Cor, Bas, and Ani exhibited
moderate antimutagenic activity; Por, Bou, and Cen exhibited
weak antimutagenic activity. For IQ in TA100, Sec and Sol
exhibited strong antimutagenic activity; Cra, Bou, and Cor
exhibited moderate antimutagenic activity; Por, Cen, Bas, and
Ani exhibited weak antimutagenic activity. For B[a]P in TA98,
Cra and Sec exhibited moderate antimutagenic activity; Cen
exhibited weak antimutagenic activity; whereas Cor, Bas, and Ani
showed no antimutagenicity, and Por, Bou, and Sol had marginal
or no antimugenic activities. For B[a]P in TA100, Cra and Sol
exhibited moderate antimutagenic activity; Por, Cor, and Ani
exhibited weak antimutagenic activity; whereas Sec and Cen
showed no effect, and Bou and Bas had marginal effect. All
samples exhibited no inhibitory effect on mutagenicity of NQNO
to TA98 and TA100, except the Sol showed strong antimutagenicity
to NQNO in TA100. Moreover, the mutagenicity of NQNO toward
TA100 was enhanced by Sec, Por, Bou, Cen, Bas, and Ani. The
antimutagenic activity of water extracts of Sec reduced after
heated at 100蚓 for 20 min. And we also found that heat-stable
antimutagens were produced in the plant extract preparation
process (homogenized, centrifuged, and freeze-dried). The water
extract of Sec was preliminary fractionated with Amicon membrane
filter. Fraction with molecular weight above 30000 showed
strongest antimutagenic acticity for Sec. Sec contained both
heat-labile and heat-stable antimutagens. The nature of the
antimutagenic components was further evaluated and compared with
their antimutagenic activity. The results suggest that
peroxidase is the major antimutagenic component in Sec. In
addition, polyphenols is one of the heat-stable antimutagens.Key
words: non-traditionally edible plants, mutagenicity,
antimutagenicity, toxicity, fractionation.
URI: http://hdl.handle.net/11455/50976
Appears in Collections:食品暨應用生物科技學系

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