Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/50980
標題: 乳酸菌抗氧化能力之探討及對脂質氧化之抑制
The antioxidative ability of lactic acid bacteria and its effects on inhibition of lipid oxidation
作者: 顏全良
Yen, Chyuan-Liang
關鍵字: antioxidants;抗氧化劑;antioxidant activity;lipid peroxidation;lactic acid bacteria;抗氧化性;脂質過氧化;乳酸菌
出版社: 食品科學系
摘要: 
乳酸菌,包括 Lactobacillus acidophilus、Lactobacillus bulgaricus
、Streptococcus thermophilus 及 Bifidobacterium longum 等 19 株
菌,以抑制抗壞血酸自氧化的測試方式,對乳酸菌是否具有抗氧化能力作
一初步篩選。在抗氧化能力之初步篩選,19 株菌的胞內物對抗壞血酸的
自氧化皆有明顯抑制,抑制率約10 %,L. acidophilus 1006 擁有最高抗
氧化性。抗氧化機制的表現,對反應性氧分子的清除、催化性金屬的螯合
、抗氧化酵素的作用及還原力的存在皆進行測試。對於反應性氧分子的清
除,19 株菌皆擁有清除氫氧自由基及過氧化氫的能力。B. longum B6 對
過氧化氫具有最強的清除能力,氫氧自由基的清除則以 L. acidophilus
E 的表現最佳。在金屬離子螯合能力的表現,L. acidophilus E、L.
acidophilus 4356 及 S. thermophilus 3641 具有螯合亞鐵離子的能力
,而 L. acidophilus N1、L. acidophilus LA-1、L. acidophilus Farr
、L. bulgaricus 12278、L. bulgaricus 448、L. bulgaricus 1006、S.
thermophilus 573、S. thermophilus 19987 及 B. longum 15708皆對銅
離子有螯合能力。抗氧化酵素的研究以超氧陰離子歧化為目標,19 株
菌無任何菌株表現出此酵素活性。19 株菌皆具還原力,B. longum B6 為
其中表現最佳者。對於具有抗氧化能力的乳酸菌株,進一步地測試其對於
脂質氧化的抑制。脂質氧化系統包括典型的不飽和脂肪酸及生物體細胞膜
。此外也探討其對於脂質毒性氧化產物及其衍生物的反應性。在脂質氧化
抑制方面,對於亞油酸乳化系統,氧化抑制率隨乳酸菌胞內物添加量的增
加而隨之提高。受測的 19 株菌中,Bifidobacterium longum B6 擁有最
強的抑制效果。對於鼠骨細胞膜系統的促氧化情況,Lactobacillus
acidophilus 4356、Lactobacillus bulgaricus 11842、Streptococcus
thermophilus 573 及 Bifidobacterium longum 15708 其胞內物的抑制
率皆超過 25 %。在脂質氧化衍生物方面,螢光組織色素為個體遭受氧化
傷害或老化程度的參考指標,4 菌株對於此色素的累積也表現出高於 20
% 的抑制率。胞內物對氫過氧化物及丙二醛等脂質氧化產物的反應,實驗
顯示乳酸菌胞內物對於低濃度 ( 10 -6 M) 的丙二醛具有分解的能力,
但對於氫過氧化物則不具反應性。

Nineteen strains of lactic acid bacteria, including
Lactobacillus acidophilus, Lactobacillus bulgaricus,
Streptococcus thermophilus, and Bifidobacterium longum were
investigated for antioxidative activity. Intracellular cell-free
extract of all strains showed antioxidative activity with
inhibition rate of ascorbate autoxidation approximately 10
%.Antioxidative mechanisms including reactive oxygen species
scavenging, metal ion chelating, enzyme inhibition, and reducing
power of cell free extract of lactic acid bacteria were studied.
All strains demonstrated reactive oxygen species scavenging
ability. B. longum B6 showed the highest hydrogen peroxide
scavenging activity and L. acidophilus E had the best hydroxyl
radical scavenging activity among the 19 strains tested. In the
chelating ability, L. acidophilus E, 4356, and S. thermophilus
3641 showed ferrous ion chelating ability. L. acidophilus N1,
LA-1, and Farr, L. bulgaricus 12278, 448, and 1006, S.
thermophilus 573, and 19987, and B. longum 15708 had cupric ion
chelating ability. Superoxide dismutase activity of 19 strains
was not found. Reducing power was found in all strains. B.
longum B6 showed the highest reducing power among the 19 strains
tested.In previous study, cell-free extract of lactic acid
bacteria demonstrated potential antioxidative activity. The
inhibition of lipid peroxidation by cell-free extract of lactic
acid bacteria was investigated by using unsaturated fatty acid
and biological membrane systems.For the inhibition of
unsaturated fatty acid peroxidation, Bifidobacterium longum B6
showed the highest antioxidative activity in linoleate emulsion
system among the 19 strains tested. In rat''s bone cell membrane
system, the cell-free extract of Lactobacillus acidophilus 4356,
Lactobacillus bulgaricus 11842, Streptococcus thermophilus 573,
and Bifidobacterium longum 15708 showed over 25% inhibition of
oxidation. These strains also showed over 20% inhibitory effect
on accumulation of fluorescent tissue pigmentThe decomposing
ability of lipid peroxidation products, t-butylhydroperoxide and
malondialdehyde, was also evaluted. The results showed that
lactic acid bacteria were not able to decompose the organic
hydroperoxide, t-butylhydroperoxide. However, malondialdehyde
was rapidly decomposed by cell-free extract of lactic acid
bacteria.
URI: http://hdl.handle.net/11455/50980
Appears in Collections:食品暨應用生物科技學系

Show full item record
 
TAIR Related Article

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.