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Development of multifunctional complex health foods and evaluation of their anti-allergic asthmatic functions using ovalbumin-sensitized and -challenged mouse model
Yu, Cheng -Yi
|關鍵字:||Allergy;過敏;Asthma;Th1/Th2 cytokines;Complex health food formula;氣喘;輔助型T細胞第一型/輔助型T細胞第二型細胞激素;組合性保健食品配方||出版社:||食品暨應用生物科技學系||摘要:||
This study attempted to develop anti-asthmatic complex food formulas. Diverse vegetable and fruit extracts were chosen as materials to prepare the basal formula A, B and C. Components analysis showed that contents of crude ash, water, total protein, total fat, total carbohydrate and phytosterol were quite different among three basal formulas. The formula C contained less amoungs of crude ash, water, total protein, total fat and phytosterol than those of formula A and B. Especially, the phytosterol level in formula C was just about 10% of formula A and B. Formulas A, B and C decreased the IL-2 secretion, hovever formulas B and C increased the IL-10 secretion by splenocytes in vitro. These results suggest that formulas A, B and C have anti-inflammatory potential.
To test their anti-asthmatic effects of these formulas A, B and C in vivo a ovalbimin (OVA) -sensitized and challenged BALB/c mice model was used. The results showed that all of three formulas decreased the cell infiltration, especially eosinophils, into bronchoalveolar lavage fluid (BALF) through five weeks tube feeding. The three formulas also decreased Th2/Th1 cytokine secretion ratios in both of BALF and splenocyte cultures, and decreased serum OVA-specific antibody IgG1 level. The results exhibited that the three formulas A, B and C have potential in immunomodulatory effects on reversing the Th2-inclination to Th1-balance in vivo. The results suggest that the three formulas are helpful against allergic asthmatic diseases.
To improve the effects of health formulas, two novel formulas, I and K were prepared according to the results of formulas A, B and C. The OVA-sensitized and -challenged BALB/c mice model was also used to test the anti-asthmatic effects of formulas I and K. The results showed that both formula I and K decreased the cell infiltration into BALF, but decreased both serum OVA-specific IgG1 and total IgE antibody levels. Besides, the formula I significantly decreased the eosinophils infiltration and Th2/Th1 cytokine secretion ratio in BALF. Inflammatory mediators eotaxin, protein and NO levels in BALF were decreased. The results exhibited that the two formulas I and K have potential against allergic asthmatic diseases. However, the effects of formula I were much better than those of formula K.
To further develop a commercial health formula, a novel formula J was prepared according to the results of formula I. The results showed that formula J decreased the cell infiltration, especially eosinophils, into BALF, and the Th2/Th1 cytokine secretion ratios in both of BALF and splenocyte cultures.The eotaxin level in BALF was also decreased. The results demonstrated that the formula J has potential in immunomodulatory effects on reversing the Th2-inclination to Th1-balance in vivo, suggesting that commercial health formula J may be helpful against allergic asthmatic diseases.
|Appears in Collections:||食品暨應用生物科技學系|
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