Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/51253
DC FieldValueLanguage
dc.contributor.advisor胡淼琳zh_TW
dc.contributor.authorLin, Li-Chuanen_US
dc.contributor.author林麗娟zh_TW
dc.contributor.other中興大學zh_TW
dc.date2007zh_TW
dc.date.accessioned2014-06-06T08:54:02Z-
dc.date.available2014-06-06T08:54:02Z-
dc.identifier.urihttp://hdl.handle.net/11455/51253-
dc.description.abstract隨著基因改造作物栽培面積的日益擴大,種類的日漸增多,許多國家都已制定了對基因改造生物(包含動植物)的管理法規,以期在上市前進行必要的安全評估工作,澄清對基因改造作物安全性之疑慮。基因改造產品之食用安全性,是以基因改造產品與其非轉殖親本間之”實質等同”(substantial equivalence)評估為基本原則;而一般成分與抗營養成分之分析,是其中必要的項目。本論文研究目的係針對國產植酸酶基因轉殖稻米(phytase-transgenic rice, PTR), 品系(event)為IGA801,與其非轉殖親本(台農67,Tainung 67, TN67),就一般成分及抗營養成分進行”實質等同性”之評估工作。其中一般成分分析項目包括:水分、灰分、粗脂肪、粗蛋白質及碳水化合物;抗營養成分分析項目包括凝集素(lectin)、植酸(phytic acid)、醇溶蛋白(prolamine)、14-16 kDa過敏原蛋白(rice allergenic protein, RAP)及胰蛋白酶抑制劑(trypsin inhibitor)等五項。樣品種類為2005年一期稻作及2006年二期稻作,2005年分為全穀(paddy rice)、糙米(brown rice)及精白米(polished rice)三種,2006年為全穀(paddy rice)一種。結果顯示:於PTR與TN67在水分、灰分、粗脂肪、粗蛋白質及碳水化合物含量具顯著性差異(p<0.05),但其數值在文獻資料之範圍內。抗營養分析之結果如下:(1) 2005及2006年之PTR與 TN67的醇溶蛋白百分比皆無顯著差異(p>0.05);(2) 2005年之全穀凝集素粗萃液對雞及兔子的紅血球凝集活性PTR顯著高於TN67,而糙米及精白米粗萃液凝集反應則是TN67顯著高於PTR; 2006年全穀之凝集素粗萃液對人類(O-Type)紅血球之凝集比活性無顯著性差異,對豬、雞、兔子之紅血球兩種樣品間具顯著性差異(p<0.05),但PTR之凝集比活性較TN67低;(3) 2005年全穀和糙米所測得之胰蛋白酶抑制劑含量PTR顯著低於TN67 (p<0.05),精白米則低於檢測極限,2006年全穀則無顯著性差異(p>0.05);(4) 2005及2006年全穀、糙米、精白米所測得之植酸含量具顯著性差異(p<0.05),但PTR之植酸均較TN67含量低;(5) 2005年PTR及TN67所含16 kDa過敏原蛋白(RAP)含量之分析,以16 kDa RAP重組蛋白製備之多株抗體測試結果:PTR所含16 kDa過敏原蛋白只有TN67之90%,顯示植酸酶基因轉入之稻米,RAP含量並無增加。綜合以上結果,國產植酸酶基因轉殖水稻,並無明顯增加上述一般成分及抗營養成分之含量。zh_TW
dc.description.abstractThe varieties and plantation acreage of genetically modified (GM) crops have increased vastly in the last decade. The food safety assessment of a GM product must be completed and proved to be substantially equivalent to its parental line (counterpart) before commercialization. The study of proximates and antinutrients is primary work for such assessment. In this study, the proximates and antinutrients of domestic phytase-transgenic rice (PTR, event IGA801) and its parental line (TN67) were analyzed and compared. The evaluated items of proximates included moisture, ash, protein, crude fat, and carbohydrates. The antinutrients assessed were lectin, phytic acid, prolamine, 14-16 kDa rice allergenic protein (RAP) and trypsin inhibitor. The paddy, brown and polished rice powders from two varieties, which were harvested in 2005 the paddy powder was collected in 2006, were subjected to assays. For proximate aspect, although several results obtained from PTR and TN67 samples were significantly different (p<0.05), these data are still within the documentary fluctuation caused by environmental shifts. The antinutrient assays showed that: (1) the quantities of prolamine obtained from two varieties were not significantly different (p>0.05); (2) Lectins extracted from 2005 paddy-rice samples of PTR exhibited higher agglutination activity to rabbit and chicken erythrocytes than did TN67. The specific agglutination activity (SAA) of lectins extracted from 2006 paddy rice samples showed no significant difference (p>0.05) for erythrocytes of human; for erythrocytes of porcine, chicken and rabbit, the SAA of lectins from PTR was significantly lower than that of TN67; (3) The quantities of trypsin inhibitor obtained from 2005 PTR were significantly lower than that of 2005 TN67; 2006 PTR and TN67 were not significantly different (p>0.05); (4) The phytic acids obtained from all PTR and TN67 samples were significantly different (p<0.05), however, the former was much lower; and (5) The quantities of 16 kDa RAP in 2005 PTR and TN67, as determined with polyclonal antibody produced by immunization of recombinant RAP, showed that the relative quantities of 16 kDa RAP from PTR was 90% of that of TN67. According to the above data, the proximates and antinutrients of PTR are considered to be substantially equivalent to its counterpart.en_US
dc.description.tableofcontents目 錄 圖次....................................................................................................... vii 表次....................................................................................................... viii 中文摘要............................................................................................... ix 英文摘要............................................................................................... xi 壹、前言.............................................................................................. 1 貳、 文獻回顧..................................................................................... 3 一、 基因改造食品之安全評估.................................................... 3 (一) 基因改造食品(Genetically Modified Foods)之定義... 3 (二) 基因改造作物的發展........................................................ 3 (三) 基因改造作物安全性的疑慮............................................ 6 (四) 風險評估............................................................................ 7 (五) 實質等同原則.................................................................... 12 二、 稻作簡介.................................................................................. 15 (一) 稻米(Rice)的介紹........................................................... 15 (二) 稻米的營養成分............................................................... 18 (三) 台農67水稻之介紹............................................................ 18 (四) 植酸酶基因轉殖水稻........................................................ 18 1. 研發背景及用途.............................................................. 19 2. 植酸酶基因轉殖水稻品系(event)IGA801的特性說明...................................................................................... 19 三、 稻米抗營養成分的介紹....................................................... 20 (一) 植酸(Phytic acid).......................................................... 20 (二) 凝集素(Lectin).............................................................. 24 (三) 胰蛋白酶抑制劑(Trypsin inhibitor)............................. 28 (四) 醇溶蛋白(Prolamine)................................................... 29 (五) 14-16 kDa過敏原蛋白(14-16 kDa rice allergenic protein)............................................................................. 30 參、 材料與方法................................................................................ 32 一、實驗架構................................................................................. 32 二、材料......................................................................................... 33 (一) 實驗材料............................................................................ 33 (二) 實驗藥品............................................................................ 33 (三) 儀器設備............................................................................ 34 三、 方法......................................................................................... 34 (一) 稻米中植酸酶活性之測定................................................. 34 (二) 一般成分分析..................................................................... 35 1. 水分.................................................................................. 35 2. 粗脂肪.............................................................................. 36 3. 粗蛋白質.......................................................................... 36 4. 灰分.................................................................................. 38 5. 碳水化合物...................................................................... 38 (三) 脂肪酸組成...................................................................... 39 (四) 胺基酸組成...................................................................... 39 (五) 抗營養成分分析.............................................................. 39 1. 植酸的測定...................................................................... 39 2. 凝集素的測定.................................................................. 41 3. 胰蛋白酶抑制劑的測定.................................................. 42 4. 醇溶蛋白的測定.............................................................. 44 5. 14-16 kDa 過敏蛋白的測定........................................... 45 (六) 統計分析方法................................................................... 45 肆、 結果與討論................................................................................ 46 一、 稻米植酸酶活性測定........................................................... 46 二、 一般成分分析....................................................................... 46 (一) 全穀稻米(Paddy rice)................................................... 46 (二) 糙米及精白米(Brown and Polished rice).................... 47 三、 脂肪酸組成........................................................................... 49 四、 胺基酸組成........................................................................... 50 五、 抗營養成分........................................................................... 51 (一) 植酸(Phytic acid).......................................................... 51 (二) 凝集素(Lectin)............................................................. 52 (三) 胰蛋白酶抑制劑(Trypsine inhibitor)........................... 54 (四) 醇溶蛋白(Prolamine)................................................... 55 (五) 14-16 kDa過敏原蛋白(14-16 kDa rice allergenic protein)............................................................................. 57 伍、 結論與展望....................................................................... 58 圖表....................................................................................................... 60 陸、參考文獻...................................................................................... 78 圖 次 圖一、基改作物安全評估程序之設計................................................. 9 圖二、基因改造作物食用安全性風險評估步驟................................. 10 圖三、非轉殖母本作物與基改作物危害特性整合性評估方法......... 11 圖四、稻米結構..................................................................................... 17 圖五、植酸化學結構............................................................................. 23 圖六、植酸酶基因轉殖稻米及其非轉殖親本植酸含量..................... 64 圖七、血球凝集反應之結果判定......................................................... 65 圖八、植酸酶基因轉殖稻米及其非轉殖親本胰蛋白酶抑制劑含量.. 71 圖九、40℃下不同溶劑萃取全穀稻米之醇溶蛋白............................. 72 圖十、60℃下不同溶劑萃取全穀稻米之醇溶蛋白............................. 73 圖十一、植酸酶基因轉殖稻米(PTR)與其非轉殖親本 (TN67)之醇溶蛋白含量................................................................................. 74 圖十二、植酸酶基因轉殖稻米(PTR)與其非轉殖親本(TN67)之醇溶蛋白含量百分比...................................................................... 75 圖十三、植酸酶基因轉殖稻米與其非轉殖親本過敏蛋白RAP含量之比較分析.............................................................................. 76 表 次 表一、2006年全球基因改造作物的面積............................................ 5 表二、不同作物中之凝集素(lectin)及其性質...................................... 27 表三、2005及2006年植酸酶基因轉殖稻米及其非轉殖親本全穀之一般成分分析............................................................................ 60 表四、2005年植酸酶基因轉殖稻米及其非轉殖親本糙米及精白米之一般成分分析........................................................................ 61 表五、植酸酶基因轉殖稻米及其非轉殖親本糙米之脂肪酸組成..... 62 表六、2005年植酸酶基因轉殖稻米及其非轉殖親本糙米之胺基酸組成............................................................................................ 63 表七、不同萃取溶液萃取全穀稻米之凝集素所含蛋白質及血球凝集效價............................................................................................ 66 表八、2005年植酸酶基因轉殖稻米及其非轉殖親本稻米凝集素萃取液對不同來源紅血球之凝集效價........................................ 67 表九、2005年植酸酶基因轉殖稻米及其非轉殖親本稻米粗萃液對不同來源紅血球之凝集比活性................................................ 68 表十、2006年植酸酶基因轉殖稻米及其非轉殖親本稻米粗萃液對不同來源紅血球之凝集效價.................................................... 69 表十一、2006年植酸酶基因轉殖稻米及其非轉殖親本稻米粗萃液對不同來源紅血球之凝集比活性.......................................... 70 表十二、2005及2006年植酸酶基因轉殖稻米及其非轉殖親本稻米之抗營養成分.......................................................................... 77zh_TW
dc.language.isoen_USzh_TW
dc.publisher食品暨應用生物科技學系zh_TW
dc.subject植酸酶zh_TW
dc.subjectphytase-transgenic riceen_US
dc.subject基因轉殖稻米zh_TW
dc.subject一般成分zh_TW
dc.subject凝集素zh_TW
dc.subject植酸zh_TW
dc.subject醇溶蛋白zh_TW
dc.subject胰蛋白酶zh_TW
dc.subject抑制劑zh_TW
dc.subject14-16 kDa過敏原蛋白zh_TW
dc.subjectproximateen_US
dc.subjectlectinen_US
dc.subjectphytic aciden_US
dc.subjectprolamineen_US
dc.subjecttrypsin inhibitoren_US
dc.subjectrice allergenic proteinen_US
dc.titleComparison of Proximates and Antinutrients of Domestic Phytase-Transgenic Rice and Its Parental Lineen_US
dc.title國產植酸酶基因轉殖稻米與其非轉殖親本一般成分及抗營養成分之比較zh_TW
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
Appears in Collections:食品暨應用生物科技學系
Show simple item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.