Please use this identifier to cite or link to this item:
Immunomodulatory inclination of various foods in immune cells and the immunomodulation of mulberry polysaccharides in OVA-sensitized murine model
|關鍵字:||過敏;Allergy;培耶氏斑細胞;脾臟細胞;多醣;樟芝;雲芝;靈芝;桑椹;草莓;splenocytes;polysaccharides;Antrodia camphorate;Coriolus versicolor;Ganoderma lucidum;mulberry;strawberry||出版社:||食品暨應用生物科技學系||摘要:||
第二部分動物體內實驗探討餵食桑椹多醣對小鼠卵白蛋白 (OVA) 特異性免疫反應之影響，結果顯示培耶氏斑細胞不論是在細胞增生、細胞激素分泌或淋巴細胞亞群變化，均不如脾臟細胞顯著，結果與體外實驗結果一致。桑椹多醣餵食組，使脾臟細胞所分泌之Th2細胞激素顯著下降，中、高劑量時顯著抑制血清IgG1之生成，降低腹腔細胞TNF-α分泌，具有抗發炎效果，整體而言，桑椹多醣對OVA-特異性免疫模式的影響，推測是經由同時調控Th1及Th2細胞的反應以及脾臟細胞分群的變化，而減緩整個過敏免疫反應。
The incidence of allergic diseases is increasing year by year. Thus, how to prevent allergic diseases and enhance immunity is concerned by people. We hypothesized that polysaccharides might exert immunomodulatory effects. Therefore, polysaccharides from three species of fungi including Antrodia camphorate, Coriolus versicolor and Ganoderma lucidum, and two species of fruits including mulberry and strawberry were selected to test their immunomodulatory effects in vitro and in vivo. In the in vitro test, the selected samples were respectively cultured with primary immune cells, including Peyer,s patches (PPs) cells and splenocytes, to evaluate their effects. In the in vivo test, polysaccharide extracts from mulberry (PEM) was chosen and conducted to an ovalbumin (OVA)-sensitized murine model.
The results from the in vitro study showed that the selected samples used in this study could not significantly affect the responses of Peyer,s patches cells, such as cell proliferation, cytokine secretions, and lymphocyte subpopulation. However, most selected samples easily stimulated splenocytes to respond. Especially, PEM administration significantly increased the cell proliferation and Th1 cytokine secretion by splenocytes. The results suggest that the administration of food components to Peyer's patches cells seem not easily to stimulate Peyer's patches cells in vitro due to the property of oral tolerance in the intestine. The cell culture model of Peyer,s patches cells in vitro could not reach the purpose of evaluating immunomodulatory food components.
The results from the in vivo study showed that supplementation with PEM for 8 weeks significantly decreased the Th2 cytokine production of splenocytes from OVA-sensitized female BALB/c mice. Furthermore, PEM administration significantly decreased serum OVA-specific IgG1 production and inhibited the secretion of tumor necrosis factor (TNF)-α by LPS-stimulated peritoneal macrophages from OVA-sensitized mice. The results suggest that PEM administration might alleviate the inflammation and hypersensitivity in allergic diseases via modulating the Th1/Th2 balance and lymphocyte subpopulation.
|Appears in Collections:||食品暨應用生物科技學系|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.