Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5189
標題: 受2,4-二硝基酚汙染土壤之生物復育
BIOREMEDIATION OF SOILS CONTAMINATED BY 2,4-DINITROPHENOL
作者: 陳炳伸
CHCHEN, P.S.
關鍵字: 生物復育;超臨界萃取;2;4-二硝基酚;2;4-DNP;BIOREMEDIATION
出版社: 環境工程學系
摘要: 
本研究之主要目的在模擬受2,4-二硝基酚(2,4-dinitrophenol,簡稱2
,4-DNP)污染之土壤在好氧和厭氧情況下進行生物復育之可行性,以及
探討利用超臨界萃取技術分析受非揮發性有機物污染土壤之可行性。研究
結果顯示,經由適當選擇超臨界萃取之操作條件(如溫度及壓力)、並建
立一系列土壤中不同2,4-DNP濃度之萃取效率以及建立收集液中2,4-DNP
含量之檢量線等超臨界萃取之必要分析條件後,可對土壤生物復育過程中
之2,4-DNP作有效之定量分析。與傳統之土壤萃取法比較,在時間之花費
及溶劑之使用上均減少許多。而在模擬受2,4-DNP污染土壤之生物復育上
,研究結果顯示,厭氧情況下之生物復育方式比好氧情況下之生物復育方
式,可處理之污染濃度較高,且生物降解速率亦隨濃度升高而加快。而在
上述二種生物復育方式下,本研究所採用之土壤現地菌對低濃度污染時,
亦具降解之能力。但若在較高污染濃度下,則須以植種方式進行生物復育
。而若提高所添加之植種菌菌量,在上述二種復育方式下,其降解速率皆
有提高之現象。另對於遭受污染之土壤在生物作用至偵測極限以下時,若
再次添加等量之污染物時,其降解速率亦有提高之現象。

The objective of this study was to discuss the possibilitiesto
treat soils contaminated by 2,4-dinitrophenol(2,4-DNP)both
in aerobic and anaerobic conditions. The possibilities to
analyze the nonvolatile organic compoundby using supercritical
fluid extraction (SFE)was alsodiscussed. The experimental
results indicated that SFEcould be effectively used to extract
organics in contaminatedsoils for GC analysis. However , the
efficiency of SFE extraction depended on the choosing the proper
operation condition of SFE(such as temperature and pressure) ,
establishing the extraction efficiency of a series of soils
contaminated by different concentrations of 2,4-DNP by SFE ,
and establishing the calculbration curves for the total amount
of 2,4-DNP in the collected solutions extracted from soils by
SFE. This process also could be used to determine the
bioremediation efficiency of 2,4-DNP contaminated soils.
Comparing the SFE with the traditional soil extraction process ,
the experimental results showed that the extraction time and the
total amount of solvent used in the extraction process could be
minimized. The results of soil bioremediation study showed that
2,4-DNP would be more effectively removed at anaerobic
condition. The results also indicated the indigenous cells
present in soils slowly degrade 2,4-DNP both in aerobic and
anaerobic environments. However , when the concentration of
2,4-DNP was relatively high , such as 20 mg/kg-soil , the
indigenous microorganisms could not effectively biodegrade 2,4-
DNP.The addition of acclimated microorganisms was required to
effectively remove 2,4-DNP at concentrations higher than 20 mg/
kg-soil.
URI: http://hdl.handle.net/11455/5189
Appears in Collections:環境工程學系所

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