Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/51994
標題: Antioxidant and anti-inflammatory activities of Equisetum spp. residues by Aspergillus carneus M34 under solid-state fermentation
Aspergillus carneus M34發酵木賊藥渣之抗氧化與抗發炎活性探討
作者: 陳欣怡
Chen, Sin-Yi
關鍵字: Equisetum spp.;木賊屬;flavonoids;Aspergillus carneus M34;solid-state fermentation;antioxidant;anti-inflammatory;anti-proliferation;類黃酮;Aspergillus carneus M34;固態發酵;抗氧化;抗發炎;抗增生
出版社: 食品暨應用生物科技學系所
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摘要: 
木賊屬含有大量生物鹼、皂素與類黃酮,其中類黃酮之強抗氧化活性於多種植物中扮演重要角色。而於發酵食品中,固態發酵為一種能增加酚類物質的含量及提升抗氧化潛力之方式。本研究主要目的為評估木賊藥渣經Aspergillus carneus M34 固態發酵後其抗氧化、抗發炎活性與抗癌細胞增生能力之變化,並探討發酵期間木賊藥渣中酚類之釋出是否與碳水化合物水解酵素有關。
實驗結果顯示,以A. carneus M34固態發酵木賊藥渣產生抗氧化物質之較適化培養條件為基質中固形物與水之比例為1:1.3;玉米浸出液1.5 g flask-1;接種量105 spores g-1;於30℃下培養8天。其較適化萃取條件為於45℃下以 99.9% 甲醇萃取發酵物,萃取時間為8小時。並以HPLC分析木賊藥渣發酵物之沖提液中,沖提液區分42及51於清除DPPH自由基能力、還原力具較好效果。
以A. carneus M34發酵木賊藥渣之產物具抗氧化活性,原因可能為alpha-amylase、xylanase、CMCase酵素活性較高之故,釋出酚類物質因而提升其抗氧化能力。
木賊藥渣發酵產物及其沖提液區分對於巨噬細胞RAW 264.7經LPS作用後可促使其生長。添加40 µg/ml發酵產物之NO抑制率可高達80.56%;沖提液區分49及56較其他沖提液區分具較好之NO抑制效果。木賊藥渣發酵產物能抑制IL-1 beta 之產量,可能之活性物質為沖提液區分56,能有效抑制IL-1 beta 生成具有劑量效應;與LPS組相比較,發酵組需於40 µg/ml沖提液區分之濃度才能明顯降低IL-6之含量(p < 0.01);添加10 µg/ml沖提液區分56則能抑制46.34%之IL-6含量(p < 0.05)。
於200 µg/ml之木賊藥渣發酵物濃度下,其AGS抑制增生率為61.46%。而於Caco-2細胞增生率方面,發酵組皆較未發酵組之增生率明顯降低,且於濃度200 µg/ml下Caco-2細胞存活率為42.54%。
綜合以上結果可知,以A. carneus M34發酵木賊藥渣可提升其抗氧化力,且抑制發炎反應,對癌細胞具有抗增生之效,具開發機能性產品之潛力。
URI: http://hdl.handle.net/11455/51994
其他識別: U0005-2206201100031200
Appears in Collections:食品暨應用生物科技學系

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