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標題: 不同研磨條件對甘草及黃柏有效成分提取及定量分析之影響
Effects of milling conditions on the extraction and analysis of Radix Glycyrrhizae Uralensis and Cortex Phellodendri extract
作者: 陳雋翰
Chen, Chun-Han
關鍵字: 中草藥;Chinese herbal materials;萃取物;微化處理;甘草;黃柏;extract;micronization;Radix Glycyrrhizae Uralensis;Cortex Phellodendri
出版社: 食品暨應用生物科技學系所
引用: 三七健康網。(2005)。認識中草藥萃取物。( 中草藥產業技術較學資源中心。(2003)。中草藥產業技術與研發。 中國植物誌。(1998)。科學出版社。北京。168。 中藥本草(上冊)。(1996)。國家中醫藥管理局中華本草編委會。上海科學技術出版社。1039-1050。 吳炫慧。2006。研究台灣與國際間可供石品使用之藥用植物及奈米食品之管理規範。碩士論文。國立中興大學。 宋健。(2001)。微膠囊化技術及應用。化學工業出版社。 李茂祥。(2006)。利用微化處理提升膳食纖維調節血脂和改善常到功能之研究。碩士論文。國立中興大學。 李鳳生、楊毅。(2002)。奈米/微米複合技術及應用。國防工業出版社,北京。 林金雀。(2002)。奈米科技市場與發展概況。工研院IEK化材組。 邱永年、張光雄。(1989)。原色台灣藥用植物圖鑑。第一卷。119。 俞小平、黃志杰。(2003)。本草綱目白化精解全書。薪傳出版社。 孫星衍、孫馮翼。(1985)。神農本草經。五洲出版社。台中。12-31。 徐國財、張立德。(2002)。奈米複合材料。化學工業出版社,北京。 馬遠榮。(2002)。奈米科技。商業周刊。台北。 常用中藥材圖鑑。(1995)。中國醫藥雜誌社主編。渡假出版社有限公司。139。 張成、賈紹義。(2009)。微波萃取技術及其應用。天津大學化工學院。 張志玲。(2004)。科學發展。375期。 張芳瑜。(2008)。利用微化處理技術改善木耳特性及調節血脂功效之研究。碩士論文。國立中興大學。 黃惠娟。(2002)。奈米入侵。商業週刊。台北。 新編中藥大辭典(下冊)。(1985)。新文豐出版社。2097-2012。 經濟部技術處。(2001)。中草藥產業現況與趨勢。 談駿嵩。(2002)。超臨界流體的應用。科技發展。359期。 薛增泉。(2002)。奈米科技探索。清華大學出版社,北京。 Abascal, K., Ganora, L., Yarnell, E., The effect of freeze-drying and its implications for botanical medicine: a review. Phytother Res. 2005, 8, 655-660. Akamatsu, H., Komura, J., Asada, Y., Niwa, Y., Mechanism of anti-inflammatory action of glycyrrhizin: effects on neutrophil functions including reactive oxygen species generation. Planta Med. 1991, 57, 119-121. Amagaya, S., Sugishita, E., Ogihara, Y., Ogawa, S., Okada, K., Aizawa, T., Comparative studies of the stereoisomers of glycyrrhetinic acid on anti-inflammatory activities. J. Pharmacobiodyn. 1984, 79, 923–928. Baker, M. E., Licorice and enzymes other than 11 betahydroxysteroid dehydrogenase: an evolutionary perspective. Steroids. 1994, 59, 136-141. Bartley, J. P., Foley, P., Supercritical fluid extraction of australian-grown ginger (Zingiber officinale). J Sci Food Agric. 1994, 66, 365-371. Belinky, P. A., Aviram, M., Furhman, B., Rosenblat, M., Vaya, J., The antioxidative effects of the isoflavan glabridin on endogenous constituents of LDL during its oxidation. Atherosclerosis. 1998, 137, 49–61. Bianchi, P. G., Petrillo, M., Lazzaroni, M., Mazzacca, G., Sabbatini, F., Piai G., Dobrilla, G., De Pretis, G., Daniotti, S., Comparison of pirenzepine and carbenoxolone in the treatment of chronic gastric ulcer. A double-blind endoscopic trial. Hepatogastroenterology. 1985, 32, 293-295. Bodmeier, R., Chen, H. G., Paeratakul, O., A novel approach to the oral delivery of micro-or nanoparticles. Pharm. Res. 1989, 6, 1469-1477. Brusq, J. M., Ancellin, N., Grondin, P., Guillard, R., Martin, S., Saintillan, Y., Issandou, M., Inhibition of lipid synthesis through activation of AMP kinase: an additional mechanism for the hypolipidemic effects of berberine. J Lipid Res. 2006, 47, 1281-1288. Chau, C. F., Wang, Y. T., Wen, Y. L., Different micronization methods significantly improve the functionality of carrot insoluble fiber. Food Chem. 2007a, 100, 1402-1408. Chau, C. F., Wen, Y. L., Wang, Y. T., Effect of micronisation on the characteristics and physicochemical properties of insoluble fibers. J. Sci. Food Agric. 2006a, 86, 2380-2386. Chau, C. F., Wen, Y. L., Wang, Y. T., Improvement of the functionality of a potential fruit insoluble fiber by micron technology. Int. J. Food Sci. Technol. 2006b, 41, 1054-1060. Chau, C. F., Wu, S. C., Lee, M. H., Physicochemical changes upon micronization process positively improve the intestinal health-enhancement ability of carrot insoluble fiber. Food Chem. 2007b, 104, 1569-1574. Cheng, Z. F., Zhang, Y. Q., Liu, F. C., Berberine against gastrointestinal peptides elevation and mucous secretion in hyperthyroid diarrheic rats Regul. Pept. 2009, 155, 145-149. Chun, M. K., Shin, H. W., Lee, H., Supercritical fluid extraction of taxol and baccatin from needle of taxus cuspidate. Biotechnol Tech. 1994, 8, 547-550. Cinatl, J., Morgenstern, B., Bauer, G., Chandra, P., Rabenau, H., Doerr, H. W., Glycyrrhizin, an active component of liquorice roots, and replication of SARS-associated coronavirus. Lancet. 2003, 361, 2045-2046. Cocking, J. B., MacCaig, J. N., Effect of low dosage of carbenoxolone sodium on gastric ulcer healing and acid secretion. Gut. 1969, 10, 219-225. Cohen, J. I., Licking latency with licorice. J Clin Invest. 2005, 115, 591-593. Conn, J. W., Rovner, D. R., Cohen, E. L., Licorice-induced pseudoaldosteronism. Hypertension, hypokalemia, aldosteronopenia, and suppressed plasma renin activity. JAMA. 1968, 205, 492-496. Cook, P. J., Vincent-Brown, A., Lewis, S. I., Perks, S., Jewell, D. P., Reed, P. I., Carbenoxolone (duogastrone) and cimetidine in the treatment of duodenal ulcer—a therapeutic trial. Scand. J. Gastroenterol Suppl. 1980, 65, 93-101. Crance, J. M., Biziagos, E., Passagot, J., Van, Cuyck-Gandre. H., Deloince, R., Inhibition of hepatitis A virus replication in vitro by antiviral compounds. J Med Virol. 1990, 31, 155-160. Curreli, F., Friedman-Kien, A. E., Flore, O., Glycyrrhizic acid alters Kaposi sarcoma-associated herpesvirus latency, triggering p53-mediated apoptosis in transformed B lymphocytes. J Clin Invest. 2005, 115, 642-652. Domadia, P. N., Bhunia, A., Sivaraman, J., Swarup, S., Dasgupta, D., Berberine Targets Assembly of Escherichia coli Cell Division Protein FtsZ. Biochemistry. 2008, 47, 3225-3234. Duke, J. A., CRC Handbook of Medicinal Herbs. CRC Press, Inc., Boca Raton, FL. 1985, 215-216. Elias R, Diazlanza AM, Vidal-Ollivier E, Maillard C, Crespin F, Balanserd G, Bondon G Influence of the drying''s process and the alcoholic degree on the extraction of hederasaponin C and α-hederin from the leaves of Hedera helix L. J. Pharm. Belg. 1991, 46, 177-181. Fanali, S., Aturlil, Z., D’Orazio, G., Raggi, M. A., Quaqlia, M. G., Sabbioni, C., Rocco, A., Use of nano-liquid chromatography for the analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and candies. J. Sep. Sci. 2005, 28, 982-986. Fenwick, G. R., Lutomski, J., Nieman, C., Liquorice, Glycyrrhiza glabra L.—Composition, uses and analysis. Food Chem. 1990, 38 119-143. Francischetti, I. M., Monteiro, R. Q., Guimaraes, J. A., Identification of glycyrrhizin as a thrombin inhibitor. Biochem Biophys Res Commun. 1997, 235, 259-263. Fuhrman, B., Buch, S., Vaya, J., Belinky, P. A., Coleman, R., Hayek, T., Aviram, M., Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation: in vitro and ex vivo studies in humans and in atherosclerotic apolipoprotein E-deficient mice. Am J Clin Nutr. 1997, 66, 276-275. Ganguli, P. C., Mohamed, S. D., Long-term therapy with carbenoxolone in the prevention of recurrence of gastric ulcer. Natural history and evolution of important side-effects and measures to avoid them. Scand. J. Gastroenterol Suppl. 1980, 65, 63-71. Garcia, G. E., Nicole, A., Bhaskaran, S., Gupta, A., Kyprianou, N., Kumar, A. P., Akt-and CREB-mediated prostate cancer cell proliferation inhibition by Nexrutine, a Phellodendron amurense extract. Neoplasia. 2006, 8, 523-533. Guillon, F., Champ, M., Structural and physical properties of dietary fibers, and consequences of processing on human physiology. Food Res. Int. 2000, 33, 233-245. Hadzic, N., Vrhovac, B., Kallai, L., Controlled double blind trial of carbenoxolone in gastric and duodenal ulcer. Int. J. Pharmacol. 1974, 10, 309-318. Haraguchi, H., Ishikawa, H., Mizutani, K., Tamura, Y., Kinoshita, T., Antioxidative and superoxide scavenging activities of retrochalchones in Glycyrrhiza inflata. Bioorg Med Chem. 1998, 6, 339-347. Haraguchi, H., Yoshida, N., Ishikawa, H., Tamura, Y., Mizutani, K., Kinoshita, T., Protection of mitochondrial functions against oxidative stresses by isoflavans from Glycyrrhiza glabra. J Pharm Pharmacol. 2000, 52, 219-223. He, J., Chen, L., Heber, D., Shi, W., Lu, Q. Y., Antibacterial compounds from Glycyrrhiza uralensis. J Nat Prod. 2006, 69, 121-124. Heaton, D. M., Bartle, K. D., Rayner, C. M., Clifford, A. A., J. High Resolut. Chromatogr. 1993, 16, 666-670. Hsu, P. K., Chien, P. J., Chau, C. F., An exploitation of the antimicrobial potential of a fruit insoluble fiber by micronization. Eur. Food Res. Technol. 2007, 225, 199-204. Hsu, P. K., Chien, P. J., Chau, C. F., Micronization increases vitamin E carrying and releasing abilities of insoluble fiber. J. Agric. Food Chem. 2008, 56, 2170-2174. Hsu, W. H., Hsieh, Y. S., Kuo, H. C., Teng, C. Y., Huang, H. I., Wang, C. J., Yang, S. F., Liou, Y. S., Kuo, W. H., Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. Arch Toxicol. 2007, 10, 719-728. Hung, Y. L., Sheu, F., Lee, M. H., Chau, C. F., Effects of particle size reduction of insoluble fibers by micron technology on various caecal and faecal indices. J. Sci. Food Agric. 2007, 88, 435-441. Iizuka, N., Miyamoto, K., Okita, K., Tangoku, A., Hayashi, H., Yosino, S., Abe, T., Morioka, T., Hazama, S., Oka, M., Inhibitory effect of Coptidis Rhizoma and berberine on the proliferation of human esophageal cancer cell lines. Cancer Lett. 2000, 1, 19-25. Inoue, H., Mori, T., Shibata, S., Koshihara, Y., Modulation by glycyrrhetinic acid derivatives of TPA-induced mouse ear oedema. Br. J .Pharmacol. 1989, 96, 204-210. Ito, M., Sato, A., Hirabayashi, K., Tanabe, F., Shigeta, S., Baba, M., De Clercq, E., Nakashima, H., Yamamoto, N., Mechanism of inhibitory effect of glycyrrhizin on replication of human immunodeficiency virus (HIV). Antiviral Res. 1988, 10, 289-298. Jang, M. H., Kim, H. Y., Kang, K. S., Yokozawa, T., Park, J. H., Hydroxyl radical scavenging activities of isoquinoline alkaloids isolated from Coptis chinensis. Arch. Pharm. Res. 2009, 32, 341-345. Jeong, H. G., You, H. J., Park, S. J., Moon, A. R., Chung, Y. C., Kang, S. K., Chun, H. K., Hepatoprotective effects of 18β-glycyrrhetinic acid on carbon tetrachloride-induced liver injury: inhibition of cytochrome P450 2E1 expression. Pharm Res. 2002, 46, 221-227. Juang, L. J., Sheu, S. J., Chemical Identification on the Origins of Phellodendri Cortex. J. Food Drug. Ana. 2004, 12, 46-51. Kase, Y., Saitoh, K., Ishige, A., Komatsu, Y., Mechanisms by which Hange-Shashin to reduce prostaglandin E2 levels. Biol Pharm Bull. 1998, 21, 1277-1281. Kawamura, F., Kikuchi, Y., Ohira, T., Yatagai, M., Accelerated Solvent Extraction of Paclitaxel and Related Compounds from the Bark of Taxus cuspidate. J Nat Prod. 1999, 62, 244-247. Kim, J. B., Lee, K. M., Ko, E., Han, W., Lee, J. E., Shin, I., Bae, J. Y., Kim, S., Noh, D. Y., Berberine inhibits growth of the breast cancer cell lines MCF-7 and MDA – MB - 231. Planta Med. 2008, 1, 39-42. Kim, S., Chung, J. H., Berberine prevents UV-induced MMP-1 and reduction of type I procollagen expression in human dermal fibroblasts. Phytomedicine. 2007, 9, 749-753. Kitagawa, I., Licorice root. A natural sweetener and an important ingredient in Chinese medicine. Pure Appl. Chem. 2002, 74, 1189-1198. Kong, W., Wei, J., Abidi, P., Lin, M., Inaba, S., Li, C., Wang, Y., Wang, Z., Si, S., Pan, H., Wang, S., Wu, J., Wang, Y., Li, Z., Liu, J., Jiang, J. D., Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat Med. 2004, 10, 1344-1351. Kroes, B. H., Beukelman, C. J., Van Den Berg, A. J. J., Wolbink, G. J., Van, Dijk. H., Labadie, R. P., Inhibition of human complement by beta-glycyrrhetinic acid. Immunology. 1997, 90, 115-120. Lee, C. H., Chen, J. C., Hsiang, C. Y., Wu, S. L., Wu, H. C., Ho, T. Y., Berberine suppresses inflammatory agents-induced interleukin-1beta and tumor necrosis factor-alpha productions via the inhibition of IkappaB degradation in human lung cells. Pharmacol Res. 2007, 3, 193-201. Leu, C. H., Li, C. Y., Yao, X., Wu, T. S., Constituents from the Leaves of Phellodendron amurense and Their Antioxidant Activity. Chem Pharm Bull (Tokyo). 2006, 54, 1308-1311. Lin, G., Nnane, I. P., Cheng, T. V., The effects of pretreatment with glycyrrhizin and glycyrrhetinic acid on the retrorsineinduced hepatotoxicity in rats. Toxicon. 1999, 37, 1259-1270. Lin, T. H., Kuo, H. C., Chou, F. P., Lu, F. J., Berberine enhances inhibition of glioma tumor cell migration and invasiveness mediated by arsenic trioxide. BMC Cancer. 2008, 8, 58-72. Mendes-Silva, W., Assafim, M., Ruta, B., Monteiro, R. Q., Guimaraes, J. A., Zingali, R. B., Antithrombotic effect of glycyrrhizin, a plant-derived thrombin inhibitor. Thrombosis Res. 2003, 112, 93-98. Miyamoto, M., Sasakawa, S., Ozawa, T., Kawaguchi, H., Ohtsuka, Y., Platelet aggregation induced by latex particles. I. Effects of size, surface potential and hydrophobicity of particles. Biomaterials. 1989, 10, 251-257. Mizutani, K., Kuramoto, T., Tamura, Y., Ohtake, N., Doi, S., Nakaura, M., Tanaka, O., Sweetness of glycyrrhetic acid 3-O-beta-D-monoglucuronide and the related glycosides. Biosci. Biotechnol. Biochem. 1994, 58, 554-555. Mukhopadhyay, M., Panja, P., A novel process for extraction of natural sweetener from licorice(Glycyrrhiza glabra)roots. Sep. Purif. Technol. 2008, 63, 539-545. Muralimanoharan, S. B., Kunnumakkara, A. B., Shylesh, B., Kulkarni, K. H., Haiyan, X., Ming, H,. Aggarwal, B. B., Rita, G., Kumar, A. P., Butanol Fraction Containing Berberine or Related Compound From Nexrutine1 Inhibits NFkB Signaling and Induces Apoptosis in Prostate Cancer Cells. Prostate. 2009, 69, 494-504. Nagy, G.S., Evaluation of carbenoxolone sodium in the treatment of duodenal ulcer. Gastroenterology 1978, 74, 7–10. Capasso, F., Mascolo, N., Autore, G., Duraccio, MR.,. Glycyrrhetinic acid, leucocytes and prostaglandins. J. Pharm. Pharmacol. 1983, 35, 332-335. Nakamura, T., Fujii, T., Ichihara, A., Enzyme leakage due to change of membrane permeability of primary cultured rat hepatocytes treated with various hepatotoxins and its prevention by glycyrrhizin. Cell Biol Toxicol. 1985, 1, 285-295. NNI(2001).History.( Nose, M., Ito, M., Kamimura, K., Shimizu, M., Ogihara, Y., A comparison of the antihepatotoxic activity between glycyrrhizin and glycyrrhetinic acid. Planta Med. 1994, 60, 136–139. Ong, E. S., Len, S. M., Pressurized hot water extraction of berberine, baicalein and glycyrrhizin in medicinal plants. Anal. Chem. Acta. 2003,482, 81-89. Ong, ES., Len, SM., Evaluation of surfactant-assisted pressurized hot water extraction for marker compounds in Radix Codonopsis pilosula using liquid chromatography and liquid chromatography/electrospray ionization mass spectrometry. J Sep. Sci. 2003, 26, 1533-1540. Onkarappa, R., Shobha, K. S., Chaya, K., Efficacy of four medicinally important plant extracts (crude) against pathogenic bacteria. Asian J Microbiol Biotech Env Sci. 2005, 7, 281-284. Pan, X. J., Liu, H. Z., Jia, G. H., Shu, Y. Y., Microwave-assisted extraction of glycyrrhizic acid from licorice root. J. Biochem. Eng. 2000, 5, 173-177. Pan, X. J., Niu, G. G., Liu, H. Z., Microwave-assisted extraction of tanshinones from Salvia miltiorrhiza bunge with analysis by high-performance liquid chromatography. J. Chromatorgr A. 2001, 922, 371-375. Park, S. D., Lai, Y. S., Kim, C.H., Immunopontentiating and antitumor activities of the purified polysaccharides from Phellodendron chinese SCHNEID.Life Sci. 2004, 75, 2621-2632. Peng, P. L., Hsieh, Y. S., Wang, C. J., Hsu, J. L., Chou, F. P., Inhibitory effect of berberine on the invasion of human lung cancer cells via decreased productions of urokinase-plasminogen activator and matrix metalloproteinase-2. Toxicol Appl Pharmacol. 2006, 1, 8-15. Richter, B. E., Jones, B. A., Ezzell, J. L., Porter, N. L., Accelerated Solvent Extraction: A Technique for Sample Preparation Anal. Chem. 1996, 68, 1033-1039. Roy, K., Mao, H. Q., Hung, S. K., Leong, K. W., Oral gene delivery with chitosan-DNA nanoparticles generates immunologic protection in a murine model of peanut allergy. Nat. Med. 1999, 5, 387-391. Sabahi, M., Mansouri, S. H., Ramezanian, M., Gholam-Hoseinian, A., Screening of plants from the southeast of Iran for antimicrobial. Int J Crude Drug Res. 1987, 25, 72-76. Sato, H., Goto, W., Yamamura, J., Kurokawa, W., Kaqeyama, S., Takahara, T., Watanabe, A., Shiraki, K., Therapeutic basis of glycyrrhizin on chronic hepatitis B. Antiviral Res. 1996, 30, 171-177. Scheneeman, B. O., Soluble and insoluble fiber-different physiological response. Food Technol. 1987, 41, 81-82. Schleimer, R. P., Potential regulation of inflammation in the lung by local metabolism of hydrocortisone. Am J Respir Cell Mol Biol. 1991, 4, 166-173. Smith, R. M., Burford, M. D., Optimization of supercritical fluid extraction of volatile constituents from a model plant matrix. J. Chromatogr. 1992, 600, 175-181. Takahara, T., Watanabe, A., Shiraki, K., Effects of glycyrrhizin on hepatitis B surface antigen: a biochemical and morphological study. J Hepatol. 1994, 21, 601-609. Tawata, M., Aida, K., Noguchi, T., Ozaki, Y., Kume, S., Sasaki, H., Chin, M., Onaya, T., Anti-platelet action of isoliquiritigenin, an aldose reductase inhibitor in licorice. Eur J Pharmacol. 1992, 212, 87-92. The Merck Index, 13th ed., Merck & Co., Rahway, NJ, 2001. Vahouny, G. V., Tombes, R., Cassidy, M. M., Kritchevsky, D., Gallo, L. L., Dietary fibers: binding of bile salts, phospholipids and cholesterol from mixed micelles by bile acid sequestrants and dietary fiber. Lipids. 1980, 15, 1012-1018. Van, Marle. J., Aarsen, PN., Lind, A., Van, Weeren-Kramer. J., Deglycyrrhizinised liquorice (DGL) and the renewal of rat stomach epithelium. Eur J Pharmacol. 1981, 72, 219-225. Van, Rossum. TGJ., Vulto, A. G., De, Man. R. A., Brouwer, J. T., Schalam, S. W., Glycyrrhizin as a potential treatment for chronic hepatitis C. Aliment Pharmacol Ther. 1998, 12, 199-205. Vaya, J., Belinky, P. A., Aviram, M., Antioxidant constituents from licorice roots: isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic Biol Med. 1997, 23, 302-313. Vinatoru, M., An overview of the ultrasonically assisted extraction of bioactive principles from herbs. Ultrason Sonochem. 2001, 8, 303-313. Walker, B. R., Edwards, C. R., 11 beta-Hydroxysteroid dehydrogenase and enzyme-mediated receptor protection: life after liquorice? Clin Endocrinol. 1991, 35, 281-289. Wang, Z. Y., Nixon, D. W., Licorice and cancer. Nutr Cancer. 2001, 39, 1-11. World Health Organization, WHO Traditional Medicine Strategy, 2002-2005 Geneva: WHO. 2000. Wu, S. C., Chien, P. J., Lee, M. H., Chau, C. F., Particle size reduction effectively enhances the intestinal health-enhancement ability of an orange insoluble fiber in hamsters. J. Food Sci. 2007, 78, 618-621. Wu, Y. L., Ling, L. D., Chau, F. T., Ultrasound-assisted extraction of ginseng saponins from ginseng roots and cultured ginesing cells. Ultrason Sonochem. 2001, 8, 347-352. Yu, S. M., Kuo, S. C., Vasorelaxant effect of isoliquiritigenin, a novel soluble guanylate cyclase activator, in rat aorta. Br J Pharmacol. 1995, 114, 1587-1594. Yu, Z., Ohtaki, Y., Kaid, K.,. Sasano, T., Shimauchi, H., Yokochi, T., Takada, H., Suqawara, S., Kumaqai, K,. Endo, Y., Critical roles of platelets in lipopolysaccharide-induced lethality: effects of glycyrrhizin and possible strategy for acute respiratory distress syndrome. Int Immunopharmacol. 2005, 5, 571-580. Zhang, B. J., Xu, D., Guo, Y., Ping, J., Chen, L. B., Wang, H., Protection by and anti-oxidant mechanism of berberine against rat liver fibrosis induced by multiple hepatotoxic factors. Clin. Exp. Pharmacol. Physiol. 2008, 3, 303-309. Zhao, S., Kwok, K. C., Liang, H., Investigation on ultrasound assisted extraction of saikosaponins form Radix Bupleuri. Sep. Purif. Technol. 2007, 55, 307-312. Zhou, J., Zhou, S., Tang, J., Zhang, K., Guang, L., Huang, Y., Xu, Y., Ying, Y., Zhang, L., Li D., Protective effect of berberine on beta cells in streptozotocin- and high- carbohydrate/high-fat diet-induced diabetic rats. Eur. J. Pharmacol. 2009, 606, 262-268.
研究結果顯示,透過球磨處理的微化方式能有效降低甘草及黃柏粉末粒徑至3.4-3.8 um,同時能夠達到省時、耗能低及高產率的萃取量效果。有關活性物質萃取效果方面,甘草經過微化處理能大幅(p < 0.05)提升其甘草酸的萃取效果,並且在常溫(25°C)、短時間(15 min)的條件下即有生藥材高溫(100°C)、長時間(120 min)相當的產量。微化處理對黃柏中小檗鹼的萃取效果在高溫的條件時,能在較短的萃取時間(15-30 min)內達到相當於生藥材組長時間的萃取量。

Preparation of Chinese herbal extract is the crucial step in the analysis of herbal and botanical industry. The present study was to investigate the effects of milling conditions on the extraction yield and analysis of both the Radix Glycyrrhizae Uralensis and Cortex Phellodendri materials. The results demonstrated that ball-milling could effectively (p < 0.05) pulverize the Glycyrrhizae and Phellodendron samples and reduce their particle sizes to 3.4-3.8 um. Meanwhile, a higher extraction yield could be achieved with micronized samples in a relatively shorter time and hence to reduce the energy consumption during extraction and drying. With respect to the extraction of bioactive compounds, micronization treatment significantly (p < 0.05) increased the extraction yield of glycyrrhizic acid from the Glycyrrhizae samples. The extraction yields of the micronized Glycyrrhizae samples under room temperature (25C) and short-time (15 min) were comparable those obtained at high temperature (100C) and long-time (120 min). Under the same temperature (100C), the extraction yields of berberine from the micronized Phellodendron samples in 15-30 min were comparable to those obtained in 120 min.
Overall, the results confirmed that appropriate micronization treatment was able to increase the yields of water-soluble extracts and bioactive compounds from the micronized Glycyrrhizae and Phellodendron samples in a shorter time and probably with lower energy consumption. These findings would be useful to exploit as well as to understand the potential applications of micronization treatment in the botanical industry as well as the herbal extraction process.
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