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標題: 芭樂心葉水萃物對STZ誘導糖尿病大鼠改善之評估
Evaluation of aqueous extract of Psidium guajava L. budding leaves on improvement of symptoms in STZ-induced SD rats
作者: 黃淑敏
Huang, Shu-Ming
關鍵字: 糖尿病;diabetes mellitus;芭樂心葉;胰臟β細胞;免疫組織化學染色;Psidium guajava L. Budding Leaves;pancreatic islets b-cells;Immunohistochemistry stain
出版社: 食品暨應用生物科技學系所
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糖尿病(diabetes mellitus)是目前全世界極力想要預防與治療的一種慢性疾病。依據行政院衛生署統計資料,國人十大死亡原因,糖尿病從1987年以後居第五位,但從2002年開始即躍升至第四位。
芭樂又名番石榴,屬桃金孃科(myrtacease),生長於中國雲南及本省台灣。台灣民間常使用芭樂果實或葉片曬乾後煮水飲用,傳說有降血壓、降血糖等功效,是一種民間盛行的草藥飲品。芭樂心葉(Psidium guajava L. Budding Leaves)是其葉片中最嫩的部份。本研究為探討芭樂心葉水萃物(Psidium guajava L. Budding Leaves Aqueous Extract, PE) 對STZ誘發糖尿病大鼠改善之評估。將SD大鼠以STZ 60 mg/kg腹腔注射誘發糖尿病,當血糖大於230 mg/dL即為誘導成功。一週後用100 mg、300 mg、1000 mg/kg三種不同劑量的芭樂心葉水萃物管餵糖尿病大鼠。於第八週後犧牲,並測量各生化指標評估其糖尿病改善之情況。由結果發現投予1000 mg/kg水萃物之組別其血糖下降明顯低於100 mg及300 mg之處理組(P < 0.05),同時胰島素分泌量也顯著增加(P < 0.05)。將取得的胰臟β細胞做組織切片並以H&E染色,發現處理組之大鼠其胰臟蘭氏小島由外觀判別與正常組有相同的情形。更進一步進行免疫組織化學染色,也發現其β細胞數量有增加的情形。此外,本研究亦發現STZ誘導之糖尿病大鼠肝臟中GSH-Px有顯著下降的情形,而SOD及Catalse則顯著上升,給予芭樂心葉處理後,其肝臟GSH-Px有上升的情形。經由研究結果推測芭樂心葉水萃物確實可以使由STZ所誘導之糖尿病大鼠增加β細胞數量,並且增加胰島素的分泌來降低高血糖,改善糖尿病症狀。

Diabetes mellitus (DM) is one of the major diseases that human being eagerly wants to overcome. In 2002, DM was the fourth of top ten causes of death in the Taiwan .
The aqueous extract of Psidium guajava L. budding leaves (PE) were tested on streptozotocin (STZ)-induced diabetic Sprague-Dawley (SD) rats. Rats (mimic DM blood sugar level > 230 mg/dL) were orally administered 100, 300, 1000 mg PE/kg body weight, respectively. PE decreased plasma sugar but increased insulin levels in a dose-dependent manner. Amylase level was significantly elevated by PE 1000 mg/kgbw (p<0.05 ). In this study was undertaken to examine the function of three antioxidant scavenger enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and Catalase (CAT). SOD and CAT activities were significantly increased in STZ rats (p<0.05), where as GSH-Px activities were decreaed. H&E stains showed that pancreatic islets and function of b-cells were partially restored by PE treatment. Results indicate that PE may alternatively act as an anti-hyperglycemic agent through regeneration of b-cell in STZ-induced DM rats.
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