Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52082
標題: Anti-enterovirus activity of Ficus pumila L.and its immune modulation
薜荔抗腸病毒活性及其免疫調節作用之研究
作者: 陳育如
Chen, Yu-Ju
關鍵字: EV71;腸病毒71型;CVB3;pro-inflammatory cytokines;Ficus pumila;克沙奇B3型;促發炎細胞激素;薜荔
出版社: 食品暨應用生物科技學系所
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Coxsackieviruses infecti -on in northern Taiwan — epidemiology and clinical characterist -iccs.J Microbiol Immunol Infect.2009, 42:38-46 Zanone M.M., Favaro E., Ferioli E., Huang G.C., Klein N.J., Perin P.C., Peakman M., Conaldi P.G., Camussi G. Human pancreatic islet endothelial cells express coxsackievirus and adenovirus receptor and are activated by coxsackie B virus infection. FASEB J.2007, 21:3308-3317
摘要: 
Outbreak of enterovirus occurred annually in Taiwan. The major isolats were enterovirus 71 (EV71), coxsackievirus B3 (CVB3), and coxsackievirus A16, which caused severe manifestations such as aseptic meningitis, cardiopulmonary edema, foot and mouth disease, meningitis, and viral myocarditis;and even death. Ficus pumila L. grows commonly in Taiwan traditionally for the treatment of kidney storing essence, antioncotic, detoxication, and blood-stanching. The aim of this study was to investigate anti-EV71 and CVB3 activity of the Ficus pumila 95% ethanol extracts and its relevant immunomodulation. The Ficus pumila 95% ethanol extract exhibited high anti-EV71 and CVB3 activity with 3.38~10.4 and 34.1~39.1 μg/mL, respectively, of 50% inhibitory concentrations (IC50). The anti-EV71 activity of the extrcet was better than that of CVB3. The Ficus pumila 95% ethanol extract had high selectivity index (SI, 23.5~72.7) against EV71. Prophylactic viral infection (inhibition rate : 0.21~5.39% and 18.1~50.4%), direct virucidal action (inhibition rate : 12.5~54.3% and 21.9~74.7%), and anti-viral replication (inhibition rate : 16.5~89.5% and 24.3~89.3%) for the extract were observed in both EV71 and CVB3 infections. The Ficus pumila 95% ethanol extract significantly increased the IL-6 and decreaced the IL-8, IL-2 and IL-1β (only effective on RD infected cells) levels in the EV71-or CVB3-infected cells. A high correlation was observed between the extract and the IL-6 increasing. The IL-8 and IL-1β reduction is able to retard the EV71 or CVB3-induced inflammation. To summarize, the Ficus pumila 95% ethanol extract exhibited high anti-EV71 and CVB3 activity, in which the IL-6 plays an important role in antivirus.

台灣每年春夏季節均會爆發腸病毒感染,分離之病毒株主要為腸病毒71型 (enterovirus 71, EV71)、克沙奇B3型 (coxsackievirusB3, CVB3) 及克沙奇A16型 (coxsackievirusA16, CVA16),臨床症狀包括無菌性腦膜炎、心肺水腫、手足口症及腦膜炎、病毒性心肌炎等,皆具嚴重之致命性。薜荔 (Ficus pumila L.) 為台灣野生資源極為豐富之藤本植物,傳統上具有固精消腫散毒止血之功效。本研究探討薜荔95%乙醇萃取物抗EV71及CVB3之活性及其對宿主細胞之免疫調節作用。結果顯示出,薜荔95%乙醇萃取物具有非常優異的抗EV71及CVB3活性,50%抑制濃度分別為3.38~10.4 μg/mL及34.1~39.1 μg/mL,對EV71之作用優於CVB3。薜荔萃取物對EV71具有高選擇指數 (selectivity index, SI;23.5~72.7)。萃取物對EV71及CVB3之抑制作用包括預防病毒感染 (抑制率:0.2~5.3%及18.1~50.4%)、使病毒失活 (抑制率:12.5~54.3%及25.5~74.7%) 及抑制病毒之複製 (抑制率:44.1~69.3%及10.6~85.6%)。宿主細胞免疫調節結果顯示出,薜荔95%乙醇萃取物會誘導被EV71及CVB3感染之宿主細胞顯著的增加IL-6及降低IL-8、IL-2、IL-1β (僅對感染之RD細胞) 之生成量。薜荔95%乙醇萃取物對EV71及CVB3之抑制作用與IL-6的增加有高度的相關性,IL-8、IL-1β的降低可減緩EV71及CVB3誘發之發炎反應。綜合本研究之結果,薜荔95%乙醇萃取物對EV71及CVB3顯示出高度抑制活性,其中IL-6扮演抗病毒重要角色。
URI: http://hdl.handle.net/11455/52082
Appears in Collections:食品暨應用生物科技學系

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