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標題: Construction of Baculovirus Display Vector and Its Application in Development of Canine Vaccine
作者: 劉宏仁
關鍵字: 生物技術;應用研究
The Baculovirus system has been increasingly used as an alternative to the traditional expression systems in the past decade years. The ability of the virus to display the fusion protein in native conformation presumably with all the necessary posttranslational modifications in the insect host cells can augment the immunoreactivity of the displayed antigens. In addition, the expression of the proteins through the baculovirus display system allows rapid generation of effective antigens without the need for purification of the recombinant proteins. The first generation of baculovirus display vector has been developed previously by our team. To enhance the expression level of proteins and to display multiple different proteins on cell membrane, the main objective of this study in the first year is to develop a novel baculovirus display vector that can display at least three different proteins on the baculoviral envelope or cell membrane. In the second year, a novel baculovirus display system displaying the proteins of canine distemper virus (CDV), canine parovirus virus (PV), and infectious canine hepatitis (ICH) virus, will be constructed. The potential of CDV-PV-ICH-pseudotyped baculovirus as a vaccine will be evaluated in this study.

其他識別: NSC100-2324-B005-009-CC1
Appears in Collections:分子生物學研究所

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