Development of Rapid Diagnostic Methods for Highly Pathogenic Avian Influenza Viruses

Abstract

Highly pathogenic avian influenza (HPAI) imposes a great potential threatens to the poultry industry in Taiwan, and HPAI is also a disease of zoonotic concerns. We have developed several methods for the identification of HPAI by either molecular or conventional methods. This project is proposed to develop monoclonal antibodies against H5 and H7 viruses. Once developed, these monoclonal antibodies could be used to identify H5 and H7 viruses by immuno fluorescence assay, and could also be used to identify anti-H5 and anti-H7 antibodies by Sandwich ELISA. This ELISA employs E. coli-expressed recombinant H5 and H7 proteins as antigens, and thus circumvents the danger of culture of H5 and H7 viruses. Once developed, this ELISA could be popularized, and thus simplifies and facilitates the survey of anti-H5 and H7 antibodies. This will prevent t

高病原性AI對本省養禽業者具極大之潛在危險性，並有人畜共通疾病之顧慮，本實驗室經多年努力，已建立多項能鑑定AI及其抗體之傳統或分生技術，本計畫則擬開發H5與H7之單源抗體，此單源抗除可用於免疫螢光快速鑑定AI病毒外，亦將用於開發Sandwich-ELISA或以偵測H5與H7抗血清。由於此ELISA所用之HA5與HA7抗原為E. coli所表現之重組蛋白，因此無培養H5與H7強毒之風險，將來如能開發成功，將可推廣使用，使H5與H7抗體之監控容易進行，以防止此類病毒之入侵與蔓延。