Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/52931
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dc.contributor.author林金和zh_TW
dc.contributor.other行政院國家科學委員會zh_TW
dc.contributor.other國立中興大學生命科學系(所)zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-06T08:58:03Z-
dc.date.available2014-06-06T08:58:03Z-
dc.identifierNSC97-2313-B005-020-MY2zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/52931-
dc.description.abstract文心蘭為台灣一重要之外銷蘭科切花作物,但因其對乙烯敏感性高,導致採收後小花易提早老化凋萎,造成農民之損失(Huang, 1998; Lin et al., 2004)。本研究擬經由基因工程操作來降低文心蘭切花之乙烯生成,而達到延長其瓶插壽命之目的。首先本實驗室已完成由Pseudomonas之菌株PNSL中分離出具有ACC deaminase之基因片段,並且登錄至NCBI資料庫中,登錄為#DQ830987(Maruthasalam et al., 2006)。ACC deaminase為一細菌之酵素,可針對ACC進行分解,產生α-丁酮酸(α-ketobutyric acid)以及氨(ammonia),進而有效阻斷轉殖株內生乙烯之生合成(Klee et al., 1991)。本研究擬分為兩部分:i)利用CaMV 35S 啟動子持續表現ACC deaminase ii)利用Arabidopsis thaliana AP1 (AtAP1) 啟動子使ACC deaminase專一表現於花朵。藉由基因轉殖降低文心蘭乙烯之產生,達到延長瓶插壽命之效。並減低採收後處理成本,對花卉產業有所助益,提高台灣外銷花卉產值。zh_TW
dc.description.abstractOncidium is an important orchid mainly grown for cut flower production in Taiwan especially for export purpose. Oncidium is highly sensitive to ethylene which can promote early senescence of flowers and thereby cause significant post harvest loses to the growers (Huang, 1998; Lin et al., 2004). The major objective of the present project is to reduce the endogenous ethylene levels in Oncidium cut flowers through genetic engineering so as to increase their vase-life (longevity). As a first step toward achieving this goal, from our laboratory, a gene encoding ACC deaminase was isolated from Pseudomonas strain PNSL and the sequence was submitted to NCBI data base under the accession # DQ830987 (Maruthasalam et al., 2006). ACC deaminase is a bacterial enzyme which specifically acts upon 1-aminocyclopropane-1carboxylicacid (ACC) and degrade in to equimolar amounts of α-ketobutyric acid and ammonia thus lowering endogenous ethylene level in transgenic plants (Klee et al., 1991).In this investigation, two strategies will be adapted as follows, i) constitutive expression of ACC deaminase controlled by CaMV 35S promoter and, ii) flower-specific expression of ACC deaminase controlled by Arabidopsis thaliana AP1 (AtAP1) promoter. Thus, the constitutive or flower-specific expression of ACC deaminase in the transgenic Oncidium is expected to reduce ethylene levels and thereby increase the vase-life of cut flowers. This will reduce the cost of post-harvest loses, benefit the floriculture industry, fulfill the customer needs and will ultimately boost the Oncidium cut flower export business in Taiwan.en_US
dc.language.isozh_TWzh_TW
dc.relation.urihttp://grbsearch.stpi.narl.org.tw/GRB/result.jsp?id=1730902&plan_no=NSC97-2313-B005-020-MY2&plan_year=98&projkey=PD9801-0462&target=plan&highStr=*&check=0&pnchDesc=%E5%88%A9%E7%94%A8%E5%9F%BA%E5%9B%A0%E5%B7%A5%E7%A8%8B%E5%BB%B6%E9%95%B7%E6%96%87%E5%BF%83%E8%98%AD%E5%88%87%E8%8A%B1%E7%93%B6%E6%8F%92%E5%A3%BD%E5%91%BDen_US
dc.subject基礎研究zh_TW
dc.subjectACC deaminaseen_US
dc.subject園藝, 生物技術zh_TW
dc.subjectACC deaminasezh_TW
dc.subject乙烯zh_TW
dc.subject文心蘭zh_TW
dc.subject基因轉殖zh_TW
dc.subjectEthyleneen_US
dc.subjectOncidiumen_US
dc.subjectGenetic transformationen_US
dc.title利用基因工程延長文心蘭切花瓶插壽命zh_TW
dc.titleVase-Life Improvement of Oncidium Cut Flowers through Genetic Engineeringen_US
dc.typeResearch Reportszh_TW
item.languageiso639-1zh_TW-
item.openairetypeResearch Reports-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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