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標題: 廣鹽性虱目魚抗低溫逆境機制之功能性基因體研究-新觀點之建立
New Insights into the Mechanisms of Euryhaline Milkfish upon Low-Temperature Challenge-A Functional Genomic Study
作者: 李宗翰
關鍵字: 基礎研究;milkfish;生物技術, 漁業類;虱目魚;寒害;功能性基因體學;functional gemomics;low-temperature stress

The physiological mechanisms underlying the ability of organisms to cope with changingenvironmental conditions are strong scientific interests for many researchers. Variation in the expressionof specific subsets of genes in concert with changing physiological or cellular conditions is one approachemployed by organisms to control the homeostasis of internal environment. Milkfish (Chanos chanos) isone of the essential economic species in Taiwan and they are damaged to die by cold current in winter.However, the physiological mechanisms of milkfish to cope with cold shock are less investigated.Environmental genomics which is to study the interaction of environments and genomics in organisms isan important subject for examining the environmental effects on fish physiology. The gill is an organ ofmultiple functions and directly exposed to external environments. This project, therefore, uses functionalgenomics to sift out the branchial genes which are affected by environmental temperature and to elucidatethe physiological mechanisms of milkfish coping with or damaged by low temperature. This project willaccomplish the following targets within three years: (1) to built up gill EST of milkfish and analysis ofthe data of functional genomics, (2) to investigate the cytoprotection mechanisms to cope with cold, (3) toisolate gill epidermal stem cell and use flow cytometry to study the effect of low temperature andepidermal growth factor on cell cycle, (4) to examine the effect of cold temperature on the capacity of gillosmo- and acid-base regulation, (5) to reveal the physiological significance and mechanisms ofhemoglobin in milkfish acclimated to low temperature, and (6) to investigate the effect of temperature onmilkfish growth and growth related hormone. This project uses functional genomics as basis andintegrates the approaches of cell biology, molecular biology, and physiology to illustrate a new point ofthe resistant mechanisms to cold temperature in milkfish. The results of this projects will be submitted togood journals as well as be used as the basis to prevent the damages caused by extremely cold weather inthe future.
其他識別: NSC99-2321-B005-013-MY3
Appears in Collections:生命科學系所

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