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標題: 氯離子對EBPR操作程序下SBR系統之除磷能力影響探討及菌相分析
Impact of the Chlorine Ion on a Sequencing Batch Reactor Operated Under Enhanced Biological Phosphate Removal Mode - Reactor Performance & Bacterial Community
作者: 林文宏
Lin, Wu-Hon
關鍵字: 氯離子;EBPR;生物除磷;菌相;SBR;FISH
出版社: 環境工程學系所
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本研究利用EBPR(Enhanced Biological Phosphorus Removal)程序之SBR反應槽進行植種污泥馴養,但在SBR反應槽達穩定後,發現此EBPR程序並不具有生物除磷特性。因而進行原因探討,嘗試找出影響生物除磷特性表現之可能因子。而在比較各項因子後發現,反應槽中氯離子濃度偏高和程序中氯離子濃度波動之現象,有可能為影響除磷程序下不具除磷能力之其中因素之一。後續針對氯離子進行探討,分別研究氯離子濃度波動幅度較大和氯離子濃度較低及較穩定之兩SBR系統,試圖釐清氯離子是否會對EBPR系統造成衝擊,並藉由FISH分析探討氯離子對兩系統之菌相結構所造成之影響。

Activated sludge system with alternating anaerobic and aerobic process, also being known as enhanced biological phosphate removal (EBPR) system, has been successfully applied to treat wastewater containing high concentration of phosphorus. Although these EBPR systems had been widely used in full-scale wastewater plants, poor wastewater-treating efficiency often occurs for unknown reasons.
In this study, a sequencing batch reactor (SBR) was operated under EBPR mode and was found to have poor phosphorus removal efficiency. Form the results of chemical analysis, it was suggested that the high and fluctuated concentration of chlorine in the mixed liquor could be the reason causing the failure of phosphorus removal. Subsequent experiments were performed to compare the difference on microbial structures between EBPR systems with high and low chlorine concentration. It was found that the level of anaerobically phosphorus released in system with low chlorine concentration was higher than that with high chlorine concentration. Similar results of aerobically phosphorus accumulating was also observed in these study. From the results of microbial structure analysis by using Fluorescence in situ Hybridization(FISH), it was showed that alpha- and beta-Proteobacteria were the dominated bacterial population in system influenced by a high and fluctuated chlorine concentration. It was also found that beta-Proteobacteria and Gram-positive bacteria with high GC bases content were dominated in system operated under a low and steady chlorine concentration. According to these results, alpha-Proteobacteria in this reactor may have higher salt tolerant capacity, such as chlorine, than the others microorganisms. Once the usual high salt concentration was controlled, alpha-Proteobacteria would lose its advantage to compete with others in EBPR system. Additional, most of beta-Proteobacteria existed in the system belong to the members of the β-1 and β-2-Proteobacteria which were identified as not the member of GAOs could also be responsible for the deterioration of phosphate removal efficiency in EBPR system. This group were also confirmed with relative higher tolerant capacity to salt to others in EBPR system, and doesn't lose the advantage to compete with others under low chlorine concentration.
其他識別: U0005-2208200718041300
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