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標題: 高溫菌分解有機污泥之影響因子
Factor affecting the degradation of waste activated sludge by thermophilic bacteria
作者: 翁靖媛
Weng, Jing-Yung
關鍵字: thermophilic bacteria;高溫菌;digestion
出版社: 環境工程學系所
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本研究之實驗架構主要可分為五部分,第一部分為菌株Geobacillus caldoxylosilyticus N6生理特性之初步測試,結果指出嗜高溫菌N6最適生長的溫度為65℃,且菌株在pH範圍為6.9~8.5的環境下有最佳的生長情形。若在菌株培養的過程中,希望一天即可得到胞子,則環境中最佳的MnSO4濃度為300 mg/L;而當MnSO4濃度為25 mg/L~600 mg/L時,不論何種濃度,培養三天皆可獲得大量胞子。由實驗中亦發現於Modified Thermus Medium中添加金屬離子及不同氮源,確實會對嗜高溫菌N6造成生長方面的影響,且不同濃度對菌株的影響亦不同。
第二部分為高溫消化最適操作條件之探討,由實驗結果顯示系統在消化溫度控制為65℃、pH未經任何調整、MLSS濃度為14000 mg/L、污泥於反應前先經過80℃加熱1 hr後再打碎10 min預處理的條件下,整體之去除率最佳,其MLVSS去除率可達41%。因此,在往後的實驗中,將選擇本節所測試出的最適操作條件,以期能促進污泥減量的效率。
第四部分為找出可增加消化效率的因子,實驗結果顯示當消化槽內額外添加2 mM Ca2+濃度,系統的去除效果最好,MLVSS的去除率可達50%。而Fe2+濃度對污泥減量的影響與添加濃度有關,低濃度可促進污泥分解效率;高濃度下則會產生抑制。當消化槽內所額外添加的金屬離子為Zn2+的情況下,添加濃度愈高的組別,其消化效率愈低。而不同氮源的添加實驗中,發現不論何種氮源的添加,對污泥的分解皆無幫助。

As a considerably new and dynamic technique, Thermophilic Aerobic Digestion (TAD) is highly acclaimed for its efficient sludge reduction and pathogen destruction. To determine the best operating strategies for sludge decomposition under the TAD reactor, a series of batch experiments were carried out to uncover factors affecting the degradation of waste activated sludge.
This study was composed of five main parts. The first part was a preliminary study on the characteristics of Geobacillus caldoxylosilyticus N6. Based on the experimental results, the optimum temperature for the growth of Geobacillus caldoxylosilyticus N6 was 65℃, and the range of optimum pH values was 6.9~8.5. If the concentration of MnSO4 in the medium was 300 mg/L, Geobacillus caldoxylosilyticus N6 could produce endospore after inoculating 1 day. And when concentration of MnSO4 was between 25 mg/L~600 mg/L, equally substantial amount of spore would be formed in three days after inoculation. Moreover, the addition of various metal ions and nitrogen sources also influenced the growth of Geobacillus caldoxylosilyticus N6, so does these various concentration of additives on N6.
The second part was an investigation into the optimum operating condition for Thermophilic Aerobic Digestion. The results indicated a best MLVSS removal efficiency, which was 41%, was achieved at 65℃, MLSS concentration of 14000 mg/L, and without pH control when the sludge was heated (80℃, 1 hr) and shattered (10 min) as pretreatment. Thus, in the future experiments, this could be a perspective approach for improving the efficiency of sludge decomposition.
The third part was an attempt to find factors leading to declining removal efficiency in a later period. The experiment results illustrated that the main reason lay in the inactivity of thermophilic bacteria in a later stage, accompanied by an accumulation of inhibitors in the sludge digestion tank, which hampered efficient sludge decomposition.
Then, the forth part looked into how to improve digestive efficiency. The results suggested that different concentration of additive metal ions made a difference. As the best removal efficiency of MLVSS at 50% was generated with 2 mM Ca2+ added, the addition of Zn2+ only slowed down the process, and the higher concentration of Zn2+, the lower efficiency was achieved. Also, while low Fe2+ concentration enhanced sludge biodegradability, high Fe2+ concentration only turned out a counteraction. As for adding different nitrogen sources, there was no significant influence on sludge decomposition.
Efforts were made on the final part to discern the optimal operating condition for sludge biodegradation, as well to probe into sludge from other places. Indicated by this study, the optimal operating condition also pertained to wasted activated sludge of a wastewater treatment plant in Taichung.
其他識別: U0005-2407200714031300
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