Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/53566
標題: 奈米微粒對細胞毒性篩選技術及驗証方法
作者: 闕斌如
莊秀美
關鍵字: 生物科學類, 生物技術, 基礎醫學類;Nanoparticles;奈米微粒;cytotoxicity;screening platform;細胞毒性;篩選技術;基礎研究
摘要: 
Nanomaterials and nanoparticles have received considerable attention for the past decade due to their unique properties and diverse applications in technology and life sciences. Despite the widespread use of nanomaterial products, relatively few studies have been undertaken to determine the biological effects of nanoparticle exposure. Accumulating evidences have suggested that nanoparticles exhibit cytotoxicity and genotoxicity in both in vivo and in vitro studies. Given the increasing use of nanoparticles in a range of products, it has become crucial to develop a fundamental understanding of the biological effect of nanoparticle exposure. Currently, nanoparticles toxicity testing is based on in vitro studies using cell culture system; however, many problems are found to be associated with these testing systems. The purpose of this project is to establish a fast screening platform to evaluate the cytotoxicity of nanoparticles that may expose human by inhalation, dermal absorption, and ingestion. This innovative screening platform is expected to replace traditional in vitro tests with more accuracy, higher sensitivity, and faster screening. Furthermore, similar experiments are conducted in traditional cytotoxicity assays. The comparison will be made to those different assays.

全球奈米科技正在如火如荼的發展,此種新興科技雖然在各個領域上,開拓新的希望、應用價值與新興產品,然而這些具有奈米結構的物質,其本身的化學性質與物理性質,會隨著粒徑大小的不同而有所變化,對人體的毒性危害或影響也可能有所改變,因此對於安全衛生環保亦將可能產生重大的衝擊。雖然目前已有一些關於奈米微粒毒性研究的報導,但因為各種不同的材質,製備法及毒性測試條件的不同,造成許多不一致或互相矛盾的結論出現。利用體外細胞株的實驗模式已成為最重要的檢測化學物質毒性的方法,與動物實驗模式比較的話,體外細胞株實驗提供簡單、快速、及花費經濟的優點。但在探討奈米微粒的毒性及健康風險時,由於奈米微粒的物理化學性質常限制了體外細胞株毒性的檢測方法,因傳統的檢測方法大多仰賴染劑或指示劑的運用,這些染劑或指示劑經常會與受到奈米微粒的干擾,因此發展出一套新型的具有高再現性及準確性的毒性檢測方法,就成為迫切及重要的需求。本研究團隊經過文獻回顧後也發現,國際上目前一般仍使用傳統體外細胞毒性檢測方法,來測試奈米微粒的毒性,學術界也普遍認為這些方法有缺失及不足之處,並多產生不一致的結果。本計劃旨在建立新的檢測方法,以去除傳統方法之染劑或指示劑的使用。在利用細胞連續及即時生長分析儀檢測奈米物質的細胞毒性,希望可以建立更穩定、快速及可測試大量奈米微粒樣品的篩選平台。
URI: http://hdl.handle.net/11455/53566
其他識別: EPA-100-1605-02-01
Appears in Collections:生命科學系所

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