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標題: 發展蚜蟲快速鑑定技術
Development of Quick Identification Technique for Aphids
作者: 郭美華
關鍵字: 植物保護類;應用研究;Aphid;蚜蟲;快速鑑定;技術;逢機放大多態型DNA;聚合脢鏈反應;Quick Identification;Technique;RAPD;PCR

Joining the World Trade Organization (WTO) is inevitable trend to our country under the increasing forces of free international trade. It is predictable that the frequencies of import and export trades of agricultural products will be increased dramatically thereafter; under the circumstances, the possibilities of invasion of foreign pests might be increased as well. In orders to prevent the spread of foreign pests with the transportation of agricultural products in and out, none of country in the world does not attach great importance to seek a rapid and accurate method for quarantine purpose. The main goal of this research is to apply a rapid method, namely random amplified polymorphic DNA -polymerase chain reaction (RAPD-PCR), to identify aphids on the gemonic level. In the first year research, the aims will be focused on establishing an optimal procedure to apply PAPD-PCR to identify native major aphid pests, and investigating their diversity of different populations in Taiwan. By the end of the research, we expect to set up a suitable method for identification of aphid pests, and then expend this method to set up a data bank on genomic diversities for different aphid pests in Taiwan for quarantine application.
其他識別: 89科技-6.2-檢-70
Appears in Collections:昆蟲學系

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