Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5440
標題: 空氣中總、粗、及細懸浮微粒脂溶性萃取物之雌性激素干擾效應
Estrogen disrupting activity induced by organic extracts of total,coarse,and fine particulate matter
作者: 翁仲鴻
Wong, Zhong-Hong
關鍵字: particulate matter;懸浮微粒;polyaromatic hydrocarbons;estrogen;多環芳香族碳氫化物;雌性激素
出版社: 環境工程學系所
引用: (一)中文書目 1. 陳穩至,(2000),”氣中懸浮微粒之特性與來源”國立成功大學環境工程研究所,碩士論文。 2. 陳亞嵐,(2004),”多環芳香族碳氫化合物 (PAHs) 誘發細胞毒性、核酸損壞及雌性激素干擾效應之研究”,國立中興大學環境工程研究所碩士論文。 3. 簡家宏,(2004),”神經網路於空氣品質短期預測及監測資料異常值診斷之研究-以台中縣沙鹿空品測站為例”,國立雲林科技大學環境與安全工程研究所,碩士論文。 4. 劉孟麟,(2005),”香環燃煙中PAHs成份特徵與及毒性研究”,國立成功大學環境工程研究所,碩士論文。 5. 蔡明志,(2004),“空氣污染微粒在植物表面之沈降與脫離”,國立台灣大學森林學研究所,碩士論文。 6. 林宛筠,(2002),“不同纖維方向的濾嘴對主流煙微粒收集效率之影響”,國立台灣大學職業醫學與工業衛生研究所,碩士論文。 7. 廖恬琳,(2004),“四氯戴奧辛誘導人類胚胎滋養層細胞氧化壓力及細胞之凋亡”,台北醫學大學生物醫學技術研究所,碩士論文。 8. 林伯雄、郭育良、吳勝雄、康玉薇、鄭朝璋、潘文驥、王淑惠(民90)。 化學物質干擾活體生物賀爾蒙平衡之研究。行政院環境保護署環境檢驗所專案研究計畫編號EPA-90-E3S5-02-01。 9. 林家琦(2007),”懸浮微粒誘發人類乳癌細胞(MCF-7及MDA-MB-231 cells)及肺纖維母細胞(MCR-5 cells)之毒性和DNA損害”,碩士論文, 中興大學。 10. 劉懿賢(2007),”台灣中部地區懸浮微粒之水溶性及脂溶性萃取物誘發 人類乳癌細胞脂細胞毒性和DNA氧化損害”,碩士論文,中興大學。 (二)西文書目 Douma, S.L, Husband, C., O’Donnell, M.E., Barwin, B.N., Woodend A.K. (2005). &quot;Estrogen-related Mood Disorders Reproductive Life Cycle Factors&quot;. Advances in Nursing Science 28 (4): 364–375. PMID 16292022. Fang H, Tong W, Shi LM, Blair R, Perkins R, Branham W, Hass BS, Xie Q, Dial SL, Moland CL, Sheehan DM (2001). &quot;Structure-activity relationships for a large diverse set of natural, synthetic, and environmental estrogens&quot;. Chem. Res. Toxicol. 14 (3): 280–94. doi:10.1021/tx000208y. PMID 11258977. Favreau, L. V. and Pickett, C. B.(1991).Transcriptional regulation of the rat NAD(P)H:quinine reductase gene. Identification of regulatory elements controlling basal level expression and inducible expression by planar aromatic compounds and phenolic antioxidants.J Biol Chem 266,4556-4561. Grimmer,G.,“Envrionmental Carcinogens:Polycyclic Aromatic”Environ.Sci.Technol.,v24,pp1581-1585,1983 Gunilla Andersson (2007-01-09). &quot;Bulimia May Result from Hormonal Imbalance&quot;. Karolinska Institutet. Gunther DF, Diekema DS (2006). &quot;Attenuating growth in children with profound developmental disability: a new approach to an old dilemma&quot;. Arch Pediatr Adolesc Med 160 (10): 1013–7. doi:10.1001/archpedi.160.10.1013. PMID 17018459. Hankinson,O(1995).The aryl hydrocarbon receptor complex. Annu Rev Pharmacol Toxicol 35, 307-340. Hess RA, Bunick D, Lee KH, Bahr J, Taylor JA, Korach KS, Lubahn DB (1997). &quot;A role for oestrogens in the male reproductive system&quot;. Nature 390 (6659): 509–12. doi:10.1038/37352. PMID 9393999. Hill RA, Pompolo S, Jones ME, Simpson ER, Boon WC (2004). &quot;Estrogen deficiency leads to apoptosis in dopaminergic neurons in the medial preoptic area and arcuate nucleus of male mice&quot;. Mol. Cell. Neurosci. 27 (4): 466–76. doi:10.1016/j.mcn.2004.04.012. PMID 15555924. Ho FM, Huang PJ, Lo HM, Lee FK, Chern TH, Chiu TW, Liau CS (1999) Effect ofacupuncture at nei-kuan on left ventricular function in patients with coronaryartery disease. Am J Chin Med 27:149-156. Hsieh YC, Yu HP, Frink M, Suzuki T, Choudhry MA, Schwacha MG, Chaudry IH (2007). &quot;G protein-coupled receptor 30-dependent protein kinase A pathway is critical in nongenomic effects of estrogen in attenuating liver injury after trauma-hemorrhage&quot;. Am. J. Pathol. 170 (4): 1210–8. doi:10.2353/ajpath.2007.060883. PMID 17392161. Ian Muchamore (2004-07-19). &quot;Prince Henry''s Institute - Media Release - Male sex drive linked to estrogen&quot;. Prince Henry''s Institute. J. Raloff (1997-12-06). &quot;Science News Online (12/6/97): Estrogen''s Emerging Manly Alter Ego&quot;. Science News. Retrieved on 2008-03-04. Josephson,J.,”Polycyclic aromatic hydrocarbon”Environ.Sci.Technol.,v18,pp.93A-95A,1984. Kolata, Gina (2003, Jan 9). &quot;F.D.A. Orders Warning on All Estrogen Labels&quot;. The New York Times. Retrieved on 2006-10-26. Kurzer MS (2002). &quot;Hormonal effects of soy in premenopausal women and men&quot;. J. Nutr. 132 (3): 570S–573S. PMID 11880595. Lasiuk, GC and Hegadoren, KM (2007). &quot;The Effects of Estradiol on Central Serotonergic Systems and Its Relationship to Mood in Women&quot;. Biological Research for Nursing (2007), 9 (2): 147–160. doi:10.1177/1099800407305600. PMID 17909167. Lee JM, Howell JD (2006). &quot;Tall girls: the social shaping of a medical therapy&quot;. Arch Pediatr Adolesc Med 160 (10): 1035–9. doi:10.1001/archpedi.160.10.1035. PMID 17018462. Li ST, Lozano P, Grossman DC, Graham E (2002). &quot;Hormone-containing hair product use in prepubertal children&quot;. Arch Pediatr Adolesc Med 156 (1): 85–6. PMID 11772198. Lobo FV, Cairns JA, Stolberg HO, Heggtveit HA (1989) Deathfollowing coronaryangiography in a young woman with isolated left coronary ostial stenosis.Can J Cardiol 5:149-154. Longwell,J.P.,symp.(Int.)Combust.[proc],v9,pp.1339-1350,1982 Monahan, E.C., & I.G. O''Muircheartaigh, 1986. Whitecaps and the passive remote sensing of the ocean surface, International Journal of Remote Sensing, 7, pp. 627-642. McMurry P.H. and J.C. Wilson(1983)”Droplet phase(heterogeneous) and gas phase(homogeneous) contribution to secondary ambient aerosol formation as functions of relative humidity.”J.geophys.Res. 88,pp.5101-5108. Marty,J.C.,Tissier,M.J.,Saliot,A., “Gaseous and particulate polycyclic aromatic hydrocarbons from the marine atmosphere Atmospheric Environment,v18,pp.2183-2190,1984. Massaro D, Massaro GD (2004). &quot;Estrogen regulates pulmonary alveolar formation, loss, and regeneration in mice&quot;. Am. J. Physiol. Lung Cell Mol. Physiol. 287 (6): L1154–9. doi:10.1152/ajplung.00228.2004. PMID 15298854. Menon DV, Vongpatanasin W (2006). &quot;Effects of transdermal estrogen replacement therapy on cardiovascular risk factors&quot;. Treat Endocrinol 5 (1): 37–51. doi:10.2165/00024677-200605010-00005. PMID 16396517. NLM (2006, Apr 1). &quot;IMPORTANT WARNING&quot;. Drug Information: Estrogen. MedlinePlus. Nussey and Whitehead: Endocrinology, an integrated approach, Taylor and Francis 2001. Oh DM, Phillips, TJ (2006). &quot;Sex Hormones and Wound Healing&quot;. Wounds 18 (1): 8–18. Oh WK (2002). &quot;The evolving role of estrogen therapy in prostate cancer&quot;. Clin Prostate Cancer 1 (2): 81–9. PMID 15046698. Paulson, K.E.,Darnell,J.E.,Jr.,Rushmore, T.,and Pickett,C.B(1990).Analysis of the upstream elements of the xenobiotic compound-inducible and positionally regulated glutathione S-transferase Ya gene.Mol Cell Biol 10,1841-1852. Prossnitz ER, Arterburn JB, Sklar LA (2007). &quot;GPR30: A G protein-coupled receptor for estrogen&quot;. Mol. Cell. Endocrinol. 265-266: 138–42. doi:10.1016/j.mce.2006.12.010. PMID 17222505. Rothenberg, Carla J. (2005-04-25). &quot;The Rise and Fall of Estrogen Therapy: The History of HRT&quot; Sanghavi, DM (October 17, 2006), “Preschool Puberty, and a Search for the Causes”,The New York Times, <http://www.nytimes.com/2006/10/17/science/17puberty.html>. Tapiero,H., Ba,G.N. and Tew,K.D. (2002) Estrogens andEnvironmental estrogens. Biomed.Pharmacother., 56, 36-44. Tremollieres FA, Pouilles JM, Cauneille C, Ribot C (1999) Coronary heart diseaserisk factors and menopause: a study in 1684 French women. Atherosclerosis142:415-423. U.S. Environmental Protection Agency (1998) The Inventory of Sources of Dioxin in the United States,Draft, EPA/600/P-98/002Aa. U.S. Environmental Protection Agency(2000)Exposure and Human Health Reassessment of 2,3,7,8-Tetrachlorodibenzo-p-Dioxin(TCDD)and Related Compounds, Part I:Estimating Exposure to Dioxin-Like Compounds, Volume 3: Properties , Environmental Levels, and Background Exposures, EPA/600/P-00/001Bc. Whitby,KT.,Svedrvp,G.M.(1980),”California aerosol:Their Physical and Chemical Characteristics”,Advanced Environment Science Technology,Vol.10,pp.477. Warnock JK, Swanson SG, Borel RW, Zipfel LM, Brennan JJ (2005). &quot;Combined esterified estrogens and methyltestosterone versus esterified estrogens alone in the treatment of loss of sexual interest in surgically menopausal women&quot;. Menopause 12 (4): 374–84. doi:10.1097/01.GME.0000153933.50860.FD. PMID 16037752. Whitlock.J.P.,Jr(1990)Genetic and molecular aspects of 2,3,7,8-tetrachlorodibenzo-p-dioxin action. Annu Rev Pharmacol Toxicol 30,251-277. Zandere,M.,”Physical and Chemical Properties of Polycyclic Aromatic Hydrocarbons”,Marcel Dekker,Inc.,1985. Zheng,M.,Fang,M., Wang, F., and To, K.L., 2000, “Characterization of the solvent extractable organic compounds in PM2.5 aerosols in Hong Kong”Atmos.Environ.,Vol.34,pp.2691-2702.
摘要: 
懸浮微粒(particulate matter, PM)會引起不利於人類廣泛的生理影響,長期的暴露於高濃度的懸浮微粒中人類易好發肺癌及乳癌的環境致病因子,本研室在先前的研究調查中已論證懸浮微粒有機萃取物,其中包含多環芳香族碳氫化物(polyaromatic hydrocarbons, PAHs)和戴奧辛(dioxins)為PM引起氧化壓力的一個重要的角色而伴隨而來的是引起人類乳癌細胞的DNA傷害,而類戴奧辛(dioxins-like)化合物為中斷體內雌性激素平衡的來源,藉由PM的影響而讓我們知道PM的有機萃取物引起類雌性激素作用的機制,主要的研究目的為懸浮微粒可能引起人類乳癌細胞(T47D-Kbluc)的雌性激素及抗雌性激素作用,為了達成此目標,我們應用了生化的偵測方式去檢測藉由人類乳癌細胞暴露在PM有機萃取物下所起引的細胞增殖作用和以report gene的方法顯示,細胞增殖分析結果表示細微粒(PM2.5)、粗微粒(PM10)及總微粒(TSP)依濃度不同而引起有相對T47D-Kbluc明顯數量的增生,這些可見細胞暴露於PM有機萃取物中比起控制組可造成2-3倍之多的增生,此在指標性基因(reporter gene)的方法比起控制組也顯示出一個顯著的指標基因酵素活性上升(~20-fold),更近一步地研究指出藉由PM有機萃取物暴露於抗雌性激素受體,ICI-182,780,可完全地抑制細胞增生作用與在指標性基因的方法亦顯著顯示,總括而言,本研究結果可提供關鍵的資訊就是在PM環境中所暴露不同粒徑微粒關連性與隨之而來的引發人類乳癌細胞的類雌性激素之增生作用,我們探討在中台灣所採樣的三個收集站之微粒脂溶性萃取物在人類乳癌細胞瓶內實驗中,具有顯著的雌性激素干擾效應。

Particulate matters (PM) are known to induce a broad-spectrum of adverse biological effects in humans. Chronic exposure to high levels of PM are environmental etiological agents of lung and breast cancer in humans. Previous investigation in our laboratory demonstrated that organic solvent-extractable compounds from PM, including polycyclic aromatic hydrocarbons (PAHs) and dioxins, play an important role in PM-induced oxidative stress and the subsequent induction of DNA lesions in human breast cancer cells. As dioxin-like compounds are known to be the source of disruption of estrogen homeostasis, investigation into the induction of estrogen-like activity by organic solvent extracts of PM should shed light on the mechanism(s) by which PM exert their actions. The primary goal of this study is to examine whether exposure to the organic extracts of PM may induce estrogenic and anti-estrogenic effects in human T47D-Kbluc breast cancer cells. To achieve these research goals, we applied biochemical assays to assess the induction of cell proliferation and the expression of estrogen receptor-mediated luciferase reporter gene by the organic extracts of PM in T47D-Kbluc cells. Results from cell proliferation analyses indicated that the organic extracts of fine particles (PM2.5), coarse particles (PM10), and total suspended particles(TSP) induced concentration-dependent increases in the number of viable cells in T47D cells. The number of viable cells in cells treated with organic extracts of PM was 2-3-fold greater than that of controls. The data also indicated that a significant increase (~20-fold) in luciferase activity was detected in cells treated with organic extracts of fine particles, coarse particles, and TSP when compared to controls. Further investigation indicated that the antiestrogen, ICI-182,780, was able to completely inhibit the induction of cell proliferation and luciferase expression induced by organic extracts of PM. Overall, results from this research should provide pivotal information of the relevance of the environmental exposure to different diameter of PM and the subsequent induction of estrogen-like activity in human T47D breast cancer cells. We concluded that the organic solvent extracts of fine particles collected at three sampling sites in central Taiwan are capable of inducing the most pronounced estrogenic effect via estrogen receptor-mediated gene expression in human breast cancer cells.
URI: http://hdl.handle.net/11455/5440
其他識別: U0005-2101200905531600
Appears in Collections:環境工程學系所

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.