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Developing Detecting Probes for Orchids Plant-Parasitic Nematodes
The orchid industry in Taiwan has great advantages in the international trading market. Theorders from the orchid festival held in Taiwan were exceeding 1 billion NT dollars for thelast few years. Orchid growers had found stylet-bearing nematodes from the orchid roots, theplanting moss and the irrigation water from their plantations. The preliminary survey resultsindicated that most orchid farms had plant parasitic nematodes, and the morphological datashowed these nematodes belong to Aphelenchoides genus. These plant parasitic nematodeswere suspected to be brought in to the farm via irrigation water or planting materials, but noconclusive evidences were found to support this theory. The previous control experimentsusing seven nematicides did not lower the nematodes to the none-detectable quarantine level;some would even cause flower deform and the orchid lost its economic value. Preventionwould be the best and economical tactic to manage this problem. Many plant parasiticnematodes are important quarantine pests, the licensed orchid farms were allowed to exportthe orchids with planting materials to USA, therefore, could not afford any risks for theexistence of quarantine nematodes in the exporting orchids. A rapid and efficient method todetect the plant parasitic nematodes on the orchids is necessary. Our research team had themost complete collection of Aphelenchoides isolates and their molecular data in Taiwan.These advantages would help shorting the process of developing the detecting probs. Theresults of this project could also help companies that have biochip developing skill toproduce a new product, which could help orchid farmers to rapidly screening their plantingmaterials, including irrigation water for the presence of the quarantine nematodes. The newproducts would also be a great advantage for our government to have for the quarantinepurpose.
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