Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/54888
標題: 以辣椒為模式探討寄主抗炭疽病之相關基因體分析(II)
Genome Analysis of Anthracnose-Resistance Using Hot Pepper as the Model Host (II)
作者: 張碧芳
關鍵字: 應用研究;cDNA-amplified Fragment Length Polymorphism (cDNA-AFLP);植物保護類, 生物技術;互補DNA-增幅片段長度多型性;防禦相關基因;炭疽病;炭疽病菌;差異展示-反轉錄-聚合酵素連鎖反應;番椒屬;辣椒;Defense-related Genes;Anthracnose;Colletotrichum acutatum;Differential Display-reverse Transcript -polymerase Chain Reaction (DD-RT-PCR);Capsicum spp;Hot Pepper
摘要: 
目前台灣地區之作物栽培受到炭疽病(Anthracnose)危害嚴重,炭疽病之常見病原菌有Colletotrichum acutatum、Co. gloeosporioides及Co. capsici等。而辣椒(Capsicum annuum)為國內主要香辛作物之一,炭疽病更是辣椒著果期的重要病害之一,對果實損害極劇,嚴重影響其經濟價值。至今尚無經濟、有效的方法來防除上述病害,也無抗病的商業品種可供利用,故若能培育出同時抗多種病害之辣椒品種(系),將是最有效且治本的防治措施。因此本研究計畫目的在於研究辣椒中之抗炭疽病相關基因表現情形與寄主辣椒本身抗病機制之關連性,並選殖辣椒的抗病相關基因,以便將來研究該些防禦基因之功能,以作為後續抗病機制研究、抗病育種篩選標的之參考,甚至可以利用遺傳工程技術開發出可持續表現此基因且對病原菌具有抗性之轉基因辣椒。本研究以世界蔬菜中心的抗病辣椒種原(Ca. chinense AVRDC PBC932)果實,利用同一種但具不同強弱毒力的炭疽病菌株 [同為Co. acutatum,編號各為Coll-365(弱毒力)、Coll-153(中毒力)及Coll-524(強毒力)] 進行微注射(microinjection)接種,並收取接種後一至三天之辣椒果實,以抽取總量RNA。再以「差異展示-反轉錄-聚合酵素連鎖反應(differential display-reverse transcript-polymerase chain reaction, DD-RT-PCR)」與「互補DNA-增幅片段長度多型性(cDNA-amplified fragment length polymorphism, cDNA-AFLP)」技術篩選出可能參與抗病反應之辣椒基因。本研究成果對茄科作物之抗病品種開發,或抗病機制之研究將有助益,並期能在植物防疫及農業生產方面有所貢獻。

Anthracnose, caused by Colletotrichum acutatum, Co. gloeosporioides, and Co. capsici, is one of the major pepper (Capsicum annuum) fruit diseases. So far, no effective measures could be applied for disease control. Breeding for anthracnose-resistant hot pepper cultivars could be the best strategy for disease management. In this study, we try to isolate genes involved in resistance to Co. acutatum. The World Vegetable Center (the former Asian Vegetable Research and Development Center) had isolated some high virulent (i.e. Coll-524), moderate virulent (i.e. Coll-153), and low virulent isolates (i.e. Coll-365) of Co. acutatum. These fungal isolates will be used to inoculate the resistant pepper fruits (AVRDC PBC932, Ca. chinense) by microinjection to study the factors involved in pepper anthracnose resistance. The genes involved in anthracnose resistance will be screened by using differential display-reverse transcript-polymerase chain reaction (DD-RT-PCR) and cDNA-amplified fragment length polymorphism (cDNA-AFLP) techniques. These genes will be cloned and analyzed for their functions, involvement in anthracnose resistance, and be further used for breeding program as screening markers, genetic materials, or even as targets for transgenic pepper or Solanaceous crops.
URI: http://hdl.handle.net/11455/54888
其他識別: 98農科-9.2.4-檢-B6(1)
Appears in Collections:動物科學系

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