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dc.contributor.advisorC-hih-Jen Luen_US
dc.contributor.authorShen, Yu-Tzuen_US
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Jegatheesan (2007) “Landfill leachate treatment using thermophilic membrane bioreactor” Desalination 204:8-16 Vogelaar, J. C. T., A. Klapwijk, J. B. V. Lier, and W. H. Rulkens (2000) “Temperature effects on the oxygen transfer rate between 20 and 55℃” Wat. Res. 34:1037-1041 Yao, C., S. Tang, Z. He, and X. Deng (2005) “Kinetics of lipase-catalyzed hydrolysis of olive oil in AOT/isooctane reversed micelle” J. Molecular Catalysis B: Enzymatic 35:108-112 Yilmaz, T., A. Yuceer, and M. Basibuyuk (2008) “A comparison of the performance of mesophilic and thermophilic anaerobic filters treating papermill wastewater” Bioresource Technol. 99:156-163zh_TW
dc.description.abstract摘 要 本研究以桃園縣觀音工業區某食品公司的高溫油脂污泥為菌種來源,經實驗室以小規模反應槽長期馴養後,利用純種培養技術分離純化菌株,並委託某生物科技公司將其基因序列定序,將定序結果上NCBI網站比對可能菌株,並對照傳統微生物鑑定實驗結果以確認菌株。確認菌株後,以橄欖油做為基質,利用不同基質濃度的批次實驗,探討高溫油脂分解菌的反應動力,並以實驗所得結果比對文獻中的微生物生長動力模式,探討可用於評估高溫好氧油脂分解菌的反應動力模式。 純種培養所分離純化的兩株菌,將菌種基因序列定序結果上NCBI網站比對,並與傳統微生物實驗對照後,分別為Thermus sp.與Microbacterium sp.。 以Thermus sp.進行批次實驗,基質濃度介於30 ~ 30000 mg/L TOC時,隨著基質濃度的提高Thermus sp.的µinitial亦有增加的趨勢,但當基質濃度大於3000 mg/L TOC時,µinitial呈現下降的趨勢,推測是因基質濃度高於一限制值後,高基質濃度反而會抑制微生物生長。批次實驗基質濃度為30、300、3000、30000 mg/L TOC,而µinitial分別為0.97、3.89、3.66、1.59 mg-cell/mg-cell-day。 將批次實驗結果套用文獻中的模式,利用Andrews的抑制方程式以數值分析所得結果µmax為4.95 mg-cell/mg-cell-day ,Ks為97 mg/L TOC,Ki為12000 mg/L TOC;利用變異數分析,f值為0.56,大於顯著值0.53,顯示Andrews方程式可適用於評估本研究系統中的反應動力參數。zh_TW
dc.description.abstractThe research utilized the thermophilic wasted sludge colleeded from a food company in Guanyin Industrial Park as the micbacterial resource. After the acclionation with a lab-scale reactor for more than six months, obtaining the pure culture strain of bacteria was identified through the process of gene mapping by a bio-technological company. Then, the result of was used in comparison with some possible strains of bacteria on NCBI website. The results of traditional microbial identification experiments were also used to confirm the strain of bacteria. After the identification process, olive oil was used as the substrate for a series of batch experiments at different substrate concentrations. This research employed some microbial growth kinetic models to estimates the growth and decay of the bacteria obtained from the pure culture study. Two strains of bacteria was identified from the pure culture study were identified with the results on NCBI website and the results from traditional microbial identification experiments. Two species were identified, Thermus sp. and Microbacterium sp.. Pure Thermus sp. was used as the pure culture in batch experiment at the substrate concentrations in the range of 30 to 30000 mg/L TOC. The results indicated an increase in the substrate concentrations increased the µinitial when Thermus sp. was used as the microorgomisun. When the substrate concentration was higher than 3000 mg/L TOC, µinitial significant decreased because of the effect of substrate inhibition. When the concentrations of the substrate were 30, 300, 3000, and 30000 mg/L TOC, µinitial was 0.97, 3.89, 3.66, and 1.59 mg-cell/mg-cell-day, respectively. This study used Andrews' inhibition equation to simulate the result of the batch experiments. The values of µmax 4.95 mg-cell/mg-cell-day, Ks 97 mg/L TOC, and Ki were 12000 mg/L TOC, respectively. The result showed Andrew's equation was adaptable to estimate the kinetic parameters in this system.en_US
dc.description.tableofcontents摘要 i Abstract ii 目錄 iv 表目錄 vii 圖目錄 viii 第一章 緒論 1 1-1 研究緣起 1 1-2 研究內容 2 第二章 文獻回顧 3 2-1 高溫好氧處理 3 2-1-1 高溫好氧處理定義 3 2-1-2 高溫好氧處理優點 7 2-1-3 高溫好氧處理缺點 11 2-2 油脂廢水與油脂廢水好氧分解過程 16 2-2-1 產生油脂廢水之工業 16 2-2-2 油脂的性質 17 2-2-3 油脂好氧分解 19 2-3 微生物反應動力 22 2-3-1 Michaelis – Menten Kinetics 22 2-3-2 單一基質限制生長 24 2-3-3 單一基質抑制生長 26 2-3-4 數值分析參數值求法 28 2-4 高溫菌特性 29 第三章 實驗方法 32 3-1 研究架構 32 3-2 實驗藥品 34 3-3 高溫菌種 35 3-3-1 高溫菌種來源 35 3-3-2 高溫菌之菌種分離純化 35 3-3-3 高溫菌種保存及培養方法 35 3-4 儀器設備 36 3-4-1 液相層析儀 36 3-4-2 固體總有機碳分析儀 36 3-4-3 其他儀器設備 37 3-5 實驗準備 38 3-5-1 實驗用水 38 3-5-2 實驗菌種 38 3-5-3 主要基質 38 3-5-4 無機營養鹽 38 3-5-5 液相層析儀流洗液配製 39 3-5-6 實驗物品及器具的準備與清洗 39 3-6 分析項目及方法 41 3-6-1 菌體濃度測定 41 3-6-2 脂肪分解酵素活性分析 42 3-6-3 總有機碳與溶解性有機碳分析 45 3-6-4 甲、乙、丙、丁、戊酸之分析 45 3-7 實驗流程 46 3-7-1 菌種基因序列定序 46 3-7-2 傳統微生物鑑定 46 3-7-3 高溫油脂分解菌反應動力參數實驗 54 第四章 結果與討論 56 4-1 菌種基因序列定序結果 56 4-2 傳統微生物鑑定結果 58 4-2-1 菌落特徵觀察試驗結果 58 4-2-2 染色反應試驗結果 58 4-2-3 胞外酵素活性分析試驗結果 60 4-3 菌種基因序列定序NCBI比對的可能菌種與傳統微生物鑑定對照結果 62 4-3-1 C菌株對照結果 62 4-3-2 Y菌株對照結果 62 4-4 高溫油脂分解菌生物降解批次實驗 64 4-4-1 不同基質濃度下Thermus sp. (C菌株)生長情形 64 4-5 Thermus sp.(C菌株)反應動力參數 75 4-5-1 不同油脂濃度下Thermus sp.比生長速率 75 4-5-2 Thermus sp.反應動力參數求法 77 第五章 結論與建議 81 5-1 結論 81 5-2 建議 83 第六章 參考文獻 84 附錄 菌種基因序列定序基因圖譜zh_TW
dc.subjectreaction kineticen_US
dc.subjectThermus sp.en_US
dc.subjectMicrobacterium sp.en_US
dc.subjectThermus sp.zh_TW
dc.subjectMicrobacterium sp.zh_TW
dc.titleA Kinetic Parameter Study of a Thermophilic Aerobic System Treating High Strength Oily Wasterwatersen_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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