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The Production of Phalaenopsis in Vitro by Liquid Medium (II)
Phalaenopsis seeds can germinate and grow well in liquid medium without any support. Medium depth and sowing density will be the key points for liquid culture. The medium volume used in liquid culture is less than solid culture. Therefore, the constituents of liquid medium will be very important for protocorm growth. In addition, potato had been confirmed as an important material for seed germination and growth. Moreover, it decreased the vitrification of protocorm. However, potato resulted in sticky medium, which was difficult for operation. Culture on solid medium is the main method for production of phalaenopsis seedlings at present. From sowing to transplant ex vitro, it requires twice subcultures and takes one year at least. The purpose of this research is to establish a suitable liquid culture model to replace solid culture. The increase of growth rate of seedlings and simpler procedure for phalaenopsis production will be expected.
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