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標題: Embryo Culture and Plant Regeneration from Interspecific Crosses in Arachis
作者: 胡澤寬
關鍵字: 生物技術, 農藝;應用研究
計畫目標: 探討不同濃度TDZ對不同花生種間雜交組合未熟胚誘導芽體與增殖效果, 以及種間雜種芽體及體胚再生小苗的條件.架構( 重要工作項目 ): 以花生栽培種台南十一號與野生種落花生A.monticola、A.villosa、A.cardenasii、A.pusilla、A.rigonii、A.correntina及A.spegazzinii等雜交未熟胚為材料.於清晨6點以栽培種台南十一號為母本與野生種進行雜交授粉, 同時噴施50ppm的GA , 子房柄形成且入土後噴施100ppm Kinetin.雜交後20至30天的子房按種子成熟度分為兩群, 成熟種子直接進行無菌播種, 未達子葉期之未熟種子則於27±1℃、16小時光照下, 進行胚培養.未熟胚培養: 以MS基礎培養基, 探討不同濃度2, 4-D及TDZ等對種間雜交未熟胚誘導芽體或體胚的形成及增殖效果.另以MS基礎培養基添加ascorbic acid為芽體發根培養基, 再將發根小苗進行健化.最後觀察不同花生種間雜交組合雜種之農藝性狀及染色體鑑定.預期效益: 建立種間雜交胚的拯救技術.

The objective of this project is to find a efficient protocol for regeneration of peanut interspecific hybrids from immature embryos via organogenesis or somatic embryogenesis.The immature embryos of seven crosses between cultivar ( TN11 )and wild species will be cultured on the MS media supplemented with TDZ or 2, 4-D.The formation of multiple shoots or somatic embryos, and multiplication rate will be scored after 45days culture.The regenerated shoots will be cultured on the MS medium with ascorbic acid to induce roots, and then transfer to plastic pod for hardening.The agronomic characters and chromosomes of different hybrids will also be examined.The results can be used to establish the rescue technique for interspecific hybrids.
其他識別: 90農科-2.1.1-糧-Z3(9)
Appears in Collections:農藝學系

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