利用即時聚合脢鏈鎖反應檢測飼料益生菌之技術開發(II)

Abstract

近年來益生菌在食品及飼料添加物之應用日漸廣泛, 但目前對於複雜微生物菌相中乳酸菌種之定量檢測，尚無標準及準確可靠的方法，常成為管理上的重要問題。為能有效管理此類產品及提昇畜產品品質，有必要開發本土化及進口生菌劑產品之快速及可靠的檢測技術。應用微生物分子檢測技術之即時聚合酶鏈鎖反應(Real-time PCR)除了檢測時時間大量縮短之外，目前已能達到兼顧準確度、敏感度及高效率的多項要求。本研究擬針對用於飼料之乳酸菌基因設計專一性PCR引子，配合即時聚合酶鏈鎖反應技術，建立快速定量檢測飼料生菌劑所含乳桿菌以外之乳酸菌種之方法，以期能經由分子檢測技術有效進行飼料生菌劑添加物產品的管理。

A lot of probiotic products containing lactic acid bacteria have been commercialized and used in feed industry. However, the standard and reliable detection methods for lactic acid bacteria in complicated microbial flora are still not available, and there is an urgent need to develop such methods for the accurate enumeration of commercial lactic acid bacteria intended used for probiotics in the imported or domestic feed additives.Molecular techniques for the detection of bacterial species especially the real-time polymerase chain reaction (real-time PCR) is increasing tremendously and has been proven to be a promising tool in rapid quantitative detection of bacteria with high accuracy, sensitivity and efficiency. In this study, the Real-time PCR quantitative detection technique will be developed using the PCR primers specific to the DNA sequence of non-lactobacilli lactic acid bacteria commercially used as feed probiotic additives. It is expected that the developing technique will provide rapid and accurate identification for the detection and effective management of probiotics in feed additives.