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標題: 開發改進安全級枯草桿菌及食品級乳酸菌誘導系統生產甜味蛋白質及三葉因子胜?
Development and Improvement of Gras Grade Bacillus Subtilis and Food Grade Lactococcus Lactis Inducible Systems to Produce Sweet Protein Brazzein and Intestinal Damage Repairing Peptide Trefoil Factor 1
作者: 葉娟美
關鍵字: 技術發展;食品科技;乳酸菌;枯草桿菌;食品級誘導系統;甜味蛋白;三葉因子

Food grade and GRAS grade expression systems are highly recommended in recombinant protein production by food biotechnology industry. Some difficult proteins must be expressed by inducible system to avoid the modification on DNA sequences or degraded by host protease. Lactococcus lactis is a food grade host and Bacillus subtilis ia a GRAS host. In this project, the strong constitutive expression signals which were constructed previously will be developed into inducible systems。These systems will be constructed into food grade inducible systems by using food grade selection marker nisin. Difficult proteins - recombinant sweet protein brazzein and intestinal damage repairing protein, recombinant trefoil factor 1 (TFF1) will be produced by these food grade/GRAS grade inducible systems. This project aims to improve the production level of local food biotechnology industry.
其他識別: NSC100-2313-B005-017-MY3
Appears in Collections:食品暨應用生物科技學系

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