開發改進安全級枯草桿菌及食品級乳酸菌誘導系統生產甜味蛋白質及三葉因子胜?

Abstract

食品生技產業中食品級及安全級宿主系統為重組蛋白生產重要工具。某些困難蛋白必須以誘導型系統生產，以避免被宿主修改基因序列或被蛋白酶降解。乳酸菌為食品級宿主，枯草桿菌為安全GRAS級宿主。本計畫將改造本實驗室所建構的食品級乳酸菌及安全級枯草桿菌強效持續元件為誘導型元件，配合食品級選擇標幟，建構食品級誘導系統。生產含雙硫鍵之重組甜味蛋白及具修復腸道上皮功能胜肽-重組三葉因子，期望增進安全級及食品級宿主之產能，對本土食品生物技術產業有所貢獻。

Food grade and GRAS grade expression systems are highly recommended in recombinant protein production by food biotechnology industry. Some difficult proteins must be expressed by inducible system to avoid the modification on DNA sequences or degraded by host protease. Lactococcus lactis is a food grade host and Bacillus subtilis ia a GRAS host. In this project, the strong constitutive expression signals which were constructed previously will be developed into inducible systems。These systems will be constructed into food grade inducible systems by using food grade selection marker nisin. Difficult proteins - recombinant sweet protein brazzein and intestinal damage repairing protein, recombinant trefoil factor 1 (TFF1) will be produced by these food grade/GRAS grade inducible systems. This project aims to improve the production level of local food biotechnology industry.