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標題: 紫色不含硫光合細菌與藍綠細菌共培養產氫可行性之評估
Assessing the feasibility of hydrogen production by co-cultured system combining Rhodopseudomonas palustris WP3-5 and Anabaena sp. CH3.
作者: 呂碧芬
Lu, Pi-Fen
關鍵字: Rhodopseudomonas palustris WP3-5;紫色不含硫光合菌WP3-5;Anabaena sp. CH3;co-culture;hydrogen;藍綠細菌CH3;共培養;氫氣
出版社: 環境工程學系所
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氣候環境溫室效應問題的惡化,與石化燃料的耗竭嚴重影響了人類的生活,故發展其他替代能源已成為現今重要課題。氫氣是一種乾淨且富有高能量(122 kJ/g)的永續能源,目前被視為未來主要替代能源之ㄧ,如能有效的大量生產氫氣,將會對環境有正面的幫助,現階段氫氣生產的方法為石化燃料產生氫、水電解產生氫氣與生物方法產生氫氣。
實驗先由台中某生活污水廠污泥經由熱篩程序,分離出厭氧產氫菌,並由BMP培養基增殖,作為厭氧暗醱酵槽之植種,再以廚餘進行厭氧醱酵菌半連續式產氫試驗並將其液態產物作為光合細菌連續流產氫試驗,進行兩階段生物產氫,結果顯示利用廚餘作為厭氧暗醱酵之基質能夠產氫,最高累積248ml氫氣,且富含有機酸之出流水氨氮濃度大約為5mg/L,低於紫色光合作用細菌Rhodopseudomonas plaustris WP3-5產氫時氨氮抑制濃度17mg/L,故此厭氧暗醱酵出流水適合後續光合連續流產氫試驗。利用厭氧暗醱酵出流水做為紫色不含硫光合菌產氫基質,最高氫氣產量為988.9 ml-H2/day。
第二部份以紫色光合作用細菌與藍綠菌共培養,其中紫色光合作用細菌以有機酸作為碳源產生氫氣,藍綠菌以異營方式可利用果糖作為碳源來產生氫氣,經由實驗結果得知將紫色光合作用細菌與藍綠菌共培養,可產生氫氣且藍綠菌以果糖產氫會有乙酸及其他代謝物產生,可持續提供紫色光合作用細菌利用而提高氫氣累積量。本試驗設計為將單獨培養紫色不含硫光合細菌、單獨培養藍綠細菌及共培養紫色不含硫光合細菌與藍綠細菌在同一系統條件下進行產氫批次試驗。試驗結果,單獨培養之紫色不含硫光合細菌與單獨培養之藍綠細菌個自累積產氫量相加後之總累計產氫量為56.77 ml,紫色不含硫光合細菌WP3-5與藍綠細菌CH3混合比為1:1時累積產氫量為83.04 ml,而在共培養中,當紫色不含硫光合細菌WP3-5與藍綠細菌CH3混合比為2:1時,為產氫最佳混合比,其最大氫氣累積量為140.83 ml,為兩單獨培養之總累積產氫量之2.48倍,顯示將紫色不含硫光合細菌與藍綠細菌共培養於含有機酸及果糖的培養基中,可以得到比兩者單獨培養時更多的產氫量。

Nowadays, the aggravation of greenhouse effect by combustion of non-renewable sources of energy such as fossil fuel, coal, oil, and nature gas has become a global issue. However, hydrogen can be an alternative and sustainable energy source due to the properties of clean and high energy yield(122 kJ/g), which is an environmentally friendly technology in the future. Biohydrogen, comparing with the other technologies of hydrogen production, has the advantages of organic waste recycling and cost- effectiveness. Therefore, the aim of this study focuses on the combination of three different types of microorganisms and trying to enhance the efficiency of hydrogen production. This study was divided into two parts. Firstly, after anaerobic fermentation of leftovers, the possibility of using effluent as substrate for phototrophic hydrogen production was evaluated. Secondly, the feasibility of biohydrogen production from co-culture of purple nonsulfur bacterium, Rhodopseudomonas palustris WP3-5, and heterocyst-forming filamentous cyanobacteria, Anabaena sp. CH1, was estimated.
In the first part, the inoculum of anaerobic fermentation was isolated from the sludge from wastewater treatment plant via heat treatment and BMP medium enrichment. The results show that the maximum cumulative volume of biohydrogen from semi-continuous anaerobic fermentation system is 248 ml, which is utilizing the leftovers as substrates. The results also indicate that the effluent contains large amount of volatile fatty acid but little amount of ammonium(5 mg/L). Because the ammonium concentration in effluent is lower than the inhibition threshold(17 mg/L)of Rhodopseudomonas plaustris WP3-5 during biohydrogen production, phototrophic biohydrogen system could be successfully compatible with anaerobic fermentation system as two-stage biohydrogen production system.
In the second part, the results from co-cultured system show that the maximum cumulative volume of biohydrogen reaches 140.83 ml in the WP3-5/CH1 mixed ratio of 2/1, which increases 2.48 fold comparing with the sum of cumulative volume(56.77 ml)from individual biohydrogen systems. Acetate, the metabolite of fructose via heterotrophic metabolism of Anabaena sp. CH1, could be taken by Rhodopseudomonas plaustris WP3-5 as energy source to produce biohydrogen. Therefore, the results from this study indicate that the co-culture biohydrogen system combining those two phototrophic bacteria is exactly feasible and the efficiency of biohydrogen production by co-cultured system is better than that of the single culture system.
其他識別: U0005-1707200910352600
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