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標題: 探討截留於快濾池中微生物組成及其對過濾系統造成之可能影響
Microorganism composition and its possible effect in the rapid sand filter of drinking water treatment process
作者: 郭譯惇
Kuo, Yi-Tun
關鍵字: rapid sand filter;快濾池;Microorganism composition;微生物組成
出版社: 環境工程學系所
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因此,為瞭解被截留於快濾池中之微生物組成及其對淨水程序產生之可能影響,本研究針對一般操作條件下之原水及模擬濾床中不同深度之濾砂採樣,分別利用16S rDNA及23S plastid rDNA基因序列進行PCR-DGGE分子生物技術分析,藉由定序及資料庫比對,建構出截留於淨水程序快濾池中微生物組成。微生物族群分析分為三部份討論,第一部份為易截留於濾床之微生物,以直接由濾砂上獲得之微生物族群表示,第二部份為易反洗出之族群,以由濾砂懸浮液獲得之微生物族群表示,第三部份為易培養之細菌族群,以經由Nutrient Broth (NB)培養後的細菌族群為主。藻類方面分二部份進行,第一部份針對體積較大且DNA不易萃取之矽藻,利用DAPI染色後進行顯微鏡觀察,第二部份應用藻類23S plastid rDNA基因序列進行PCR-DGGE分析,建構快濾池中之可能藻相,探討其對濾床操作之影響。本研究同時探討在添加不同濃度消毒劑下,濾床菌群結構變化,並分析胞外多醣體(EPS)濃度,已協助判斷消毒劑使用對微生物之影響。
研究結果顯示位於濾床上層微生物多樣性較豐富,中層次之,下層微生物種類較少;存在濾床中的細菌種類大部分為具有分泌胞外多醣體能力之細菌,且細菌族群受季節變化影響不大。藻類多樣性則隨季節不同而變動,經由顯微鏡觀察得知,冬季濾床突發性阻塞情形主要由矽藻(針桿藻)引起。由顯微鏡觀察結果得知當添加消毒劑不足量時,細菌及藻類會產生許多生物性凝聚物質,使針桿藻糾結在一起。配合胞外多醣體分析結果,未添加消毒劑時,各層胞外多醣體濃度平均為20 mg/L,當添加消毒劑濃度為0.3 ppm時,胞外多醣體急遽增加至42 mg/L,當消毒劑濃度為0.6 ppm時,濾床各層胞外多醣體濃度差異不大;當消毒劑濃度提高至1.2 ppm時,胞外多醣體濃度開始下降,且針桿藻糾結情形獲得改善;當消毒劑濃度添加至1.5 ppm時,胞外多醣體下降至12 mg/L,由此可知當消毒劑殺菌力不足時,可能會造成胞外多醣體增加進而影響淨水程序。
其他識別: U0005-3006200912544400
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