Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/5850
標題: 延長Rhodopseudomonas palustris WP3-5光合產氫操作策略及LED燈對光合產氫與PHB累積之影響
Strategies to prolong photo-hydrogen production of Rhodopseudomonas palustris WP3-5 for long-term operation and effects of LED light on photo-hydrogen production and PHB accumulation
作者: 古家宇
Ku, Chia-Yu
關鍵字: Rps. palustris WP3-5;Rps. palustris WP3-5;紫色不含硫光合菌;LED燈;purple non-sulfur bacteria;LED lamp
出版社: 環境工程學系所
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摘要: 
氫氣被認為是一項乾淨的能源,燃燒時只產生水與熱能(121 kJ/g),且燃燒後無排放二氧化碳之問題。而以紫色不含硫光合菌於光異營條件下進行生物產氫為具潛力的方式之一,但菌株生長速率較慢,以及光利用效率不佳之情形,且操作光合反應槽時,易被生長速率較快之異營菌污染,導致產氫速率與氫氣百分比下降。因此本研究以不同之操作策略(固定化細胞、光暗週期、共培養以及批次式進流方式),希冀能改善此缺點以提升產氫效能。另一方面,目前光合產氫多以鎢絲燈為光源,其發光時伴隨著大量熱能散失,且甚少文獻使用LED燈為光源。因此本研究仍嘗試以不同LED燈為光源,觀察對產氫及累積PHB之影響。
實驗結果發現,以固定化細胞方法進行光合產氫,固定化材質之質傳速率較差且阻礙光能穿透,因此光合菌無法利用光能進行產氫。以光暗週期方式進行光合產氫時,光暗週期之組別產氫量、細胞生長速率以及基質利用速率皆較全光照組別慢,並觀察到在黑暗期有耗氫情形發生。因此光暗週期操作之方式無法有效提升氫氣產量。將Rps. palustris WP3-5與藍綠細菌Anabaena sp. CH3以體積比1:1混合進行共培養批次試驗,glutamate濃度為50及100 mg/L時,累積氫氣量分別為43.1及45.8 ml H2。將光合反應槽操作於批次式進流,可成功延長反應槽操作時間至823(乙酸為電子供給者)及871(乳酸為電子供給者)小時並持續產氫,且受其他異營菌污染時,仍有高的氫氣百分比。
另外光合產氫批次試驗使用不同光源,結果發現以鎢絲燈為光源時,細胞生長速率、基質利用率以及產氫速率結果最快,若將光源改為LED燈,是以綠光LED燈為光源時,其細胞生長量及產氫量結果較多,但改變不同光源對於菌株WP3-5累積PHB並無影響,另外計算產生每莫耳氫氣所需之電能,若以綠光LED燈取代鎢絲燈時,可節省95%之電能。

Hydrogen is considered as an environmentally friendly fuel due to its carbon-neutral characteristic that only produces water and energy (121 kJ/g) after combustion. Using purple non-sulfur bacteria is a promising method to produce hydrogen biologically because this kind of bacteria can produce hydrogen gas under photo-heterotrophic condition. However, some problems associated with purple non-sulfur bacteria to produce hydrogen are still necessary to be solved, e.g., low cell growth rate, low conversion efficiency of light, and decreased hydrogen production rate and hydrogen content caused by heterotrophs which have high growth rate in photo-bioreactors. In this study, several strategies, including immobilized cell, diurnal-cycle operation, co-culture system and fed-batch mode operation, were tried to solve those problems which have been mentioned before and to enhance their hydrogen production. On the other hand, lots of studies used tungsten lamp as their light source for photo-hydrogen production, and it would waste high amount of energy when lighting on. Thus, second part of this study was to investigate the effect of LED light as a light source on photo-hydrogen production and PHB accumulation by Rhodopseudomoans palustris WP3-5.
From the results, it can be concluded that (i) Rps. palustris strain WP3-5 can not successfully produce hydrogen in immobilized cells because of the limitation in light penetration, substrate transportation and waste exclusion; (ii) strain WP3-5 generated less hydrogen and lower biomass under a diurnal cycle operation than continuous illumination with a hydrogen consumption by uptake hydrogenase; (iii) batch experiments of co-culture hydrogen production by Rps. palustris WP3-5/Anabaena sp. CH3 under mixed ratio of 1/1 exhibited a maximum hydrogen volume of 45.8 and 43.1 ml when co-culture medium contained 50 and 100 mg/L glutamate, respectively; (iv) fed-batch mode photo-bioreactor using acetate and lactate as electron donor can continuously produce hydrogen until 823 and 871 hours, respectively, even if heterotrophs existed in the reactor.
In addition, Rps. palustris WP3-5 had the highest cell growth rate, substrate consumption rate and hydrogen production rate when using tungsten lamp as a light source. Among various kinds of LED lamp for the other source, green-LED lamp had a higher hydrogen production rate than the other LED lamps. On the other hand, influence of light source on the PHB accumulation by strain WP3-5 was not observed. Otherwise, the energy demand to produce one mole of hydrogen gas by Rps. palustris WP3-5 was compared between the green-LED and tungsten lamps. The result showed that using green-LED lamp is of benefit to energy effiency by saving 95% of electric demand.
URI: http://hdl.handle.net/11455/5850
其他識別: U0005-2007201210005400
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