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標題: Isolation and characterization of novel giant Stenotrophomonas maltophilia phage phi SMA5
作者: Chang, H.C.
Chen, C.R.
Lin, J.W.
Shen, G.H.
Chang, K.M.
Tseng, Y.H.
Weng, S.F.
關鍵字: pseudomonas-maltophilia;cystic-fibrosis;xanthomonas;bacteriophages;infection;genome;genus;bacteremia;aeruginosa;campestris
Project: Applied and Environmental Microbiology
期刊/報告no:: Applied and Environmental Microbiology, Volume 71, Issue 3, Page(s) 1387-1393.
Stenotrophomonas maltophilia is one of the most prevalent opportunistic bacteria causing nosocomial infections. It has become problematic because most of the isolates are resistant to multiple antibiotics, and therefore, development of phage therapy has attracted strong attention. In this study, eight S. maltophilia phages were isolated from clinical samples including patient specimens, catheter-related devices, and wastewater. These phages can be divided into four distinct groups based on host range and digestibility of the phage DNAs with different restriction endonucleases. One of them, designated phi SMA5, was further characterized. Electron microscopy showed it resembled Myoviridae, with an isometric head (90 nm in diameter), a tail (90 nm long), a baseplate (25 run wide), and short tail fibers. The phi SMA5 double-stranded DNA, refractory to digestion by most restriction enzymes, was tested and estimated to be 250 kb by pulsed-field gel electrophoresis. This genome size is second to that of the largest phage, phi KZ of Pseudomonas aeruginosa. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 25 virion proteins were visualized. N-terminal sequencing of four of them suggested that each of them might have had its N terminus cleaved off. Among the 87 S. maltophilia strains collected in this study, only 61 were susceptible to phi SMA5, indicating that more phages are needed toward a phage therapy strategy. Since literature search yielded no information about S. maltophilia phages, phi SMA5 appears to be the first reported.
ISSN: 0099-2240
DOI: 10.1128/aem.71.3.1387-1393.2005
Appears in Collections:分子生物學研究所

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