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標題: Characteristic analysis of the ampC gene encoding beta-lactamase from Photobacterium phosphoreum
作者: Lin, J.W.
Weng, S.F.
Chao, Y.F.
Chung, Y.T.
關鍵字: ampC gene;AmpC (beta-lactamase);regulatory region R&R;acyl-protein synthetase;lux operon;nucleotide-sequence;bacterial;bioluminescence;escherichia-coli;vibrio-fischeri;functional-analysis;regulatory region;genome sequence;leiognathi
Project: Biochemical and Biophysical Research Communications
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 326, Issue 3, Page(s) 539-547.
The ampC gene of Photobacterium phosphoreum ATCC 11040 was cloned and identified. Nucleotide sequence of the regulatory region R&R and the ampC gene (GenBank Accession No. AY787792) from P. phosphoreum has been determined, and the encoded beta-lactamase is deduced. The beta-lactamase encoded by the ampC gene has a calculated M-r 31,198 and comprises 285 amino acid residues (pI7.35). There is a signal peptide of 20 amino acid residues MKLRFIASTLLLSFSQL (A) under bar(S) under bar(A) under bar to lead the beta-lactamase secretion, and the cleavage site is between (A) under bar(S) under bar(A) under bar -Q; thus, the matured protein only has M, 29,019 and comprises 265 amino acid residues (pI6.21). The specific amino acid residues STFK (65th to 68th), SDN (125th to 127th), and D (158th) located 33 residues downstream from the SDN loop of the class A beta-lactamases are highly conserved, but the KTG is not found. The gene order of the ampC is <--ufo-RR-ampC-->, the genes running in the opposite directions. Functional analysis elicits that R&R-[ampC] does function to lead to the gene expression. Primer extension assay elicits that the ampC gene's transcriptional initiation +1 is -26 C upstream of the start codon; the P-[1]-promoter should be the promoter response for the gene expression. Analysis of the R&R-[ampC] elicits that the upstream activator binding sequence SigmaUAS TGT (T) under bar(T) under bar(A) under bar(A) under bar(A) under bar TACGC (T) under bar(T) under bar(T) under bar(G) under bar(A) under bar ACA is like the two-component regulator binding sequence TGT-N8-12-ACA. It implies that P. phosphoreum ampC gene could be under-regulated by the specific two-component regulator. (C) 2004 Published by Elsevier Inc.
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2004.11.065
Appears in Collections:分子生物學研究所

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