Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60320
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dc.contributor.authorLin, J.W.en_US
dc.contributor.author翁淑芬zh_TW
dc.contributor.authorLin, B.J.en_US
dc.contributor.authorChen, H.Y.en_US
dc.contributor.authorWeng, S.F.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-09T05:56:14Z-
dc.date.available2014-06-09T05:56:14Z-
dc.identifier.issn0006-291Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60320-
dc.description.abstractNucleotide sequence of the luzA gene (GenBank accession No. AF039303) from Photobacterium leiognathi ATCC 25521 (NCIMB 2193) has been determined, and the chaperone encoded by the luzA gene was deduced. The LuzA chaperone has a calculated M-r 26,295 and comprises 230 amino acid residues; the hydrophobic alpha-helix N-terminal 21 amino acid residues MKKTIF-ALLFMSVFI SYPSFA is the leader peptide, therefore the matured LuzA chaperone has a calculated M-r 23,871 and comprises 209 amino acid residues only. The periplasmic LuzA chaperone is the protein concerned with the protein folding, assembly and stability. The luzA gene and the related genes are closely linked to the sad gene, that encoding Cu/Zn superoxide dismutase enables to enhance bioluminescence of the lux operon; the gene order of the luzA gene and related genes is -ufo'-LuzA-ufoI-ufoII-ter->-R&R'-sod-ufo--->. In trans complementation bioluminoassays in vivo elicit that the LuzA chaperone might be not directly concerned with bioluminescence of the lux operon from P. leiognathi in E. coli, but might enable to stabilize the proteins related to bioluminescence. The unidentified ufoII gene closely linked to the luzA gene is able to enhance bioluminescence. (C) 1998 Academic Press.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofseriesBiochemical and Biophysical Research Communications, Volume 244, Issue 3, Page(s) 838-842.en_US
dc.relation.urihttp://dx.doi.org/10.1006/bbrc.1998.8359en_US
dc.subjectacyl-protein synthetaseen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectlux operonen_US
dc.subjectbacterialen_US
dc.subjectbioluminescenceen_US
dc.subjectescherichia-colien_US
dc.subjectfunctional-analysisen_US
dc.subjectregulatoryen_US
dc.subjectregionen_US
dc.subjectvibrio-fischerien_US
dc.subjectexpressionen_US
dc.subjectcloningen_US
dc.titleCharacteristic analysis of the luzA gene encoding chaperone from Photobacterium leiognathi related to bioluminescenceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1006/bbrc.1998.8359zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:分子生物學研究所
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