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標題: UDP-glucose dehydrogenase gene of Xanthomonas campestris is required for virulence
作者: Chang, K.W.
Weng, S.F.
Tseng, Y.H.
關鍵字: pathogenicity;UDP-glucose dehydrogenase;UDP-glucuronic acid;xanthan;gum;Xanthomonas campestris;xylE reporter;syringae pv tomato;xanthan gum;extracellular polysaccharide;pseudomonas-aeruginosa;filamentous phages;dna;pigmentation;pathogenesis;expression;insertion
Project: Biochemical and Biophysical Research Communications
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 287, Issue 2, Page(s) 550-555.
Xanthomonas campestris pv. campestris (Xc) is the casual agent of black rot in crucifers. The Xc gene (udgH) coding for UDP-glucose dehydrogenase, an enzyme catalyzing the conversion of UDP-glucose to UDP-glucuronic acid, was previously shown to be required for the biosynthesis of xanthan gum, a substance necessary for the bacterium to cause disease. In this study, the transcription start site of the udgH was determined and the promoter activity monitored by the xylE reporter system indicated that expression of the udgH increases following cell growth and that the udgH gene may possess a second promoter that is responsive to stationary-phase change retaining high levels of expression. Results of Southern hybridization suggest that the udgH gene may be ubiquitous in Xanthomonas, coincident with the notion that members of this genus are capable of xanthan gum biosynthesis. Mutation of the udgH gene in Xc and X. campestris pv. vesicatoria, the casual agent of leaf spot in pepper and tomato, was found to cause a loss of virulence. (C) 2001 Academic Press.
ISSN: 0006-291X
DOI: 10.1006/bbrc.2001.5591
Appears in Collections:分子生物學研究所

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