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|標題:||The early stages of filamentous phage phi Lf infection require the host transcription factor, Clp||作者:||Lee, T.C.
|關鍵字:||campestris pv campestris;amp receptor protein;utilizing;xanthomonas-campestris;circle dna-replication;cyclic-amp;escherichia-coli;salmonella-typhimurium;vibrio-cholerae;cloning;vectors;xanthan gum||Project:||Journal of Molecular Microbiology and Biotechnology||期刊/報告no：:||Journal of Molecular Microbiology and Biotechnology, Volume 3, Issue 3, Page(s) 471-481.||摘要:||
Xanthomonas campestris pv, campestris produces great amounts of an exopolysaccharide (EPS), xanthan gum. Eight eps loci involved in biosynthesis of the EPS were previously located in the chromosome map of strain Xc17, In this study, the eps8 region was cloned, sequenced and found to contain a crp homologue whose deduced amino acid sequence possesses similarity to that of the cyclic AMP receptor protein of bacteria, with the highest identity (97%) being shared with the X, campestris pv, campestris B-1459 clp gene previously shown to be involved in pathogenicity and regulation of the production of xanthan, extracellular enzymes, and pigment (de Crecy-Lagard V., Glaser P., Lejeune P,, Sismeiro O., Barber C.E., Daniels M.J., and Danchin A., J, Bacteriol, 172:5877-5883, 1990), Based on sequence identity, pleiotropic effects of the mutation, the ability to complement an Escherichia coli cya crp mutant, and Southern hybridization detecting a single copy in the chromosome, we propose this eps8 gene to be the Xc17 clp, In addition to the previously reported properties, a clp mutant (AU56E) cannot be plagued with filamentous phage phi Lf, although it retains the capability to support phi Lf DNA replication and release authentic phage particles upon electroporation of the RF DNA, Infective center assays demonstrated that the frequency of infection is 460- to 7,500-fold lower in AU56E compared to that in the wild-type Xc17, Electron microscopy, which showed no surface appendages other than the monotrichous flagellum, confirmed that AU56E drastically diminishes in the efficiency of phage adsorption. These results suggest Clp to be regulating the biosynthesis of the primary receptor, most likely a type IV pilus, Upstream to clp is a homologue of the E. coli speD gene required for spermidine synthesis. Mutation of the clp flanking regions and transcriptional analyses suggest clp to be monocistronic and the only gene contained at the eps8 locus.
|Appears in Collections:||分子生物學研究所|
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