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標題: Isolation and Characterization of a Novel Lysine Racemase from a Soil Metagenomic Library
作者: Chen, I.C.
Lin, W.D.
Hsu, S.K.
Thiruvengadam, V.
Hsu, W.H.
關鍵字: acylamino acid racemase;dependent dna-ligase;d-amino acids;semialdehyde dehydrogenase;bacillus-subtilis;crystal-structure;escherichia-coli;proline racemase;d-aspartate;enzymes
Project: Applied and Environmental Microbiology
期刊/報告no:: Applied and Environmental Microbiology, Volume 75, Issue 15, Page(s) 5161-5166.
A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and D-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the L-lysine-to-D-lysine direction than in the reverse reaction.
ISSN: 0099-2240
DOI: 10.1128/aem.00074-09
Appears in Collections:分子生物學研究所

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