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http://hdl.handle.net/11455/60333
標題: | Isolation and Characterization of a Novel Lysine Racemase from a Soil Metagenomic Library | 作者: | Chen, I.C. 許文輝 Lin, W.D. Hsu, S.K. Thiruvengadam, V. Hsu, W.H. |
關鍵字: | acylamino acid racemase;dependent dna-ligase;d-amino acids;semialdehyde dehydrogenase;bacillus-subtilis;crystal-structure;escherichia-coli;proline racemase;d-aspartate;enzymes | Project: | Applied and Environmental Microbiology | 期刊/報告no:: | Applied and Environmental Microbiology, Volume 75, Issue 15, Page(s) 5161-5166. | 摘要: | A lysine racemase (lyr) gene was isolated from a soil metagenome by functional complementation for the first time by using Escherichia coli BCRC 51734 cells as the host and D-lysine as the selection agent. The lyr gene consisted of a 1,182-bp nucleotide sequence encoding a protein of 393 amino acids with a molecular mass of about 42.7 kDa. The enzyme exhibited higher specific activity toward lysine in the L-lysine-to-D-lysine direction than in the reverse reaction. |
URI: | http://hdl.handle.net/11455/60333 | ISSN: | 0099-2240 | DOI: | 10.1128/aem.00074-09 |
Appears in Collections: | 分子生物學研究所 |
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