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標題: The IS1 elements in Shigella boydii: horizontal transfer, vertical inactivation and target duplication
作者: Hsu, W.B.
Chen, J.H.
關鍵字: IS1;transposition activity;target duplication;Shigella boydii;Escherichia coli O157 : H7;Escherichia coli K12;insertion-sequence is1;escherichia-coli;nucleotide-sequence;variable;length;cloning vectors;genome sequence;transposition;specificity;flexneri;strains
Project: Fems Microbiology Letters
期刊/報告no:: Fems Microbiology Letters, Volume 222, Issue 2, Page(s) 289-295.
IS1(SB) and its two variants were identified as the major and minor IS1 elements in Shigella boydii. The nucleotide sequences of IS1(SB), IS1(O157:H7) from Escherichia coli O157:H7 and IS1F from E coli K12 suggest that these IS1 elements had been horizontally transferred among S. boydii and E coli O157:H7 and K12. The two ISI(SB) variants and ISI(O157:H7) have transposition activities 7- to 86-fold less than that of IS1(SB), whereas IS1F has little transposition activity. Analysis of the flanking sequences of IS1(SB) and its two variants in S. boydii revealed the nature of regional specificity of the target sites and the sequence dependence of 8 and 9 bp target duplications, for which a model is presented. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(03)00319-7
Appears in Collections:分子生物學研究所

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