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dc.contributor.authorShen, G.H.en_US
dc.contributor.authorChen, C.H.en_US
dc.contributor.authorHung, C.H.en_US
dc.contributor.authorWu, K.M.en_US
dc.contributor.authorLin, C.F.en_US
dc.contributor.authorSun, Y.W.en_US
dc.contributor.authorChen, J.H.en_US
dc.description.abstractSETTING: Many hospitals in Taiwan use the fully automated BACTEC Mycobacterial Growth Indicator Tube (MGIT) 960 system to identify mycobacteria in clinical specimens, while the labour-intensive BD ProbeTec ET (CTB) system or biochemical methods are used to identify Mycobacterium tuberculosis complex (MTC) in mycobacterially positive BACTEC cultures. OBJECTIVE: To evaluate whether the Capilia TB assay can be used to replace the BD ProbeTec ET (CTB) system or biochemical methods for identifying MTC in BACTEC cultures. DESIGN: Mycobacterially positive BACTEC cultures were collected and MTC in the cultures was identified using biochemical methods. MTC was identified using serpentine cording in smears, the Capilia TB assay or the BD ProbeTec ET (CTB) system, and the results were compared. RESULTS: Using 23 3 mycobacterially positive BACTEC cultures, the sensitivity and specificity of identification of the Capilia TB assay were respectively 96.9% and 98.6%, while those of the BD ProbeTec ET (CTB) system were respectively 99.4% and 97.3%. Combining the Capilia TB assay with serpentine cording in smears led to 100% specificity for intersected results and 100% sensitivity for combined results. CONCLUSION: The Capilia TB assay can he used to identify MTC in BACTEC cultures. By combining the assay with serpentine cording in smears, false-positives and -negatives may be reduced.en_US
dc.relationInternational Journal of Tuberculosis and Lung Diseaseen_US
dc.relation.ispartofseriesInternational Journal of Tuberculosis and Lung Disease, Volume 13, Issue 3, Page(s) 371-376.en_US
dc.subjectMycobacterium tuberculosis complexen_US
dc.subjectCapilia TB assayen_US
dc.subjectBD ProbeTec ETen_US
dc.subject(CTB) systemen_US
dc.subjectserpentine corden_US
dc.subjectrapid presumptive identificationen_US
dc.subjectbactec mgit 960en_US
dc.subjectculture confirmationen_US
dc.subjectmicroscopic morphologyen_US
dc.subjectavium complexen_US
dc.subjectbd probetecen_US
dc.titleCombining the Capilia TB assay with smear morphology for the identification of Mycobacterium tuberculosis complexen_US
dc.typeJournal Articlezh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
Appears in Collections:分子生物學研究所
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