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標題: Purification and expression of the gene III protein from filamentous phage phi Lf
作者: Liu, T.J.
You, B.Y.
Lin, N.T.
Yang, M.T.
Tseng, Y.H.
關鍵字: campestris pv campestris;single-stranded-dna;xanthomonas-campestris;nucleotide-sequence;adsorption protein;escherichia-coli;genome;fd;bacteriophages;replication
Project: Biochemical and Biophysical Research Communications
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 242, Issue 1, Page(s) 113-117.
The gene III protein (pIII) from phi Lf, a filamentous phage of Xanthomonas campestris pv. campestris, was purified by gel filtration with FPLC. The gIII coding region was amplified by PCR, which was then cloned into pUC18 and expressed in Escherichia coli. The size of both pIII, purified from phage particle and expressed in E. coli, is similar to the value deduced from the nucleotide sequence as shown by Western blot analysis. This is different from the case in Ff phages (f1, fd, and M13), in which the size of pIII observed in SDS-polyacrylamide gel electrophoresis is substantially larger than the deduced value. Upon infection of X. c. pv. vesicatoria carrying cloned phi LfgIII with phi Xv, a filamentous phage of pv. vesicatoria, the progeny particles in supernatant were able to infect both pv. campestris carrying cloned phi LfgIII and pv. vesicatoria, indicating that a mixture of authentic phi Xv and chimeric phage consisting of phi Xv DNA and phi LfpIII was produced. These results suggest pIII to be the adsorption protein required for host recognition. (C) 1998 Academic Press.
ISSN: 0006-291X
DOI: 10.1006/bbrc.1997.7932
Appears in Collections:分子生物學研究所

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