Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60368
DC FieldValueLanguage
dc.contributor.authorLiu, T.J.en_US
dc.contributor.author楊明德zh_TW
dc.contributor.authorYou, B.Y.en_US
dc.contributor.authorLin, N.T.en_US
dc.contributor.authorYang, M.T.en_US
dc.contributor.authorTseng, Y.H.en_US
dc.date1998zh_TW
dc.date.accessioned2014-06-09T05:56:19Z-
dc.date.available2014-06-09T05:56:19Z-
dc.identifier.issn0006-291Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60368-
dc.description.abstractThe gene III protein (pIII) from phi Lf, a filamentous phage of Xanthomonas campestris pv. campestris, was purified by gel filtration with FPLC. The gIII coding region was amplified by PCR, which was then cloned into pUC18 and expressed in Escherichia coli. The size of both pIII, purified from phage particle and expressed in E. coli, is similar to the value deduced from the nucleotide sequence as shown by Western blot analysis. This is different from the case in Ff phages (f1, fd, and M13), in which the size of pIII observed in SDS-polyacrylamide gel electrophoresis is substantially larger than the deduced value. Upon infection of X. c. pv. vesicatoria carrying cloned phi LfgIII with phi Xv, a filamentous phage of pv. vesicatoria, the progeny particles in supernatant were able to infect both pv. campestris carrying cloned phi LfgIII and pv. vesicatoria, indicating that a mixture of authentic phi Xv and chimeric phage consisting of phi Xv DNA and phi LfpIII was produced. These results suggest pIII to be the adsorption protein required for host recognition. (C) 1998 Academic Press.en_US
dc.language.isoen_USzh_TW
dc.relationBiochemical and Biophysical Research Communicationsen_US
dc.relation.ispartofseriesBiochemical and Biophysical Research Communications, Volume 242, Issue 1, Page(s) 113-117.en_US
dc.relation.urihttp://dx.doi.org/10.1006/bbrc.1997.7932en_US
dc.subjectcampestris pv campestrisen_US
dc.subjectsingle-stranded-dnaen_US
dc.subjectxanthomonas-campestrisen_US
dc.subjectnucleotide-sequenceen_US
dc.subjectadsorption proteinen_US
dc.subjectescherichia-colien_US
dc.subjectgenomeen_US
dc.subjectfden_US
dc.subjectbacteriophagesen_US
dc.subjectreplicationen_US
dc.titlePurification and expression of the gene III protein from filamentous phage phi Lfen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1006/bbrc.1997.7932zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:分子生物學研究所
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