Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60370
標題: A novel restriction-modification system from Xanthomonas campestris pv. vesicatoria encodes a m4C-methyltransferase and a nonfunctional restriction endonuclease
作者: Yu, Y.J.
楊明德
Yang, M.T.
關鍵字: restriction-modification system;Xanthomonas;pseudogene;dna cytosine methyltransferase;escherichia-coli;sequence;gene;enzymes;cloning;genome;n4-methylcytosine;recognition;pseudogenes
Project: Fems Microbiology Letters
期刊/報告no:: Fems Microbiology Letters, Volume 272, Issue 1, Page(s) 83-90.
摘要: 
A novel restriction-modification (R-M) system, designated as xveIIRM, from chromosomal DNA of the Xanthomonas campestris pv. vesicatoria. strain 7-1 (Xcv7-1) was cloned and characterized. The xveIIRM genes involved in this R-M system are aligned in a tail-to-tail orientation and overlapped by 12 base pairs. XveII methyltransferase gene could encode a 299-amino acid protein (M.XveII) with an estimated mass of 33.7 kDa and was classified to be a member of P-class of m4C-MTase. M.XveII methylates the second cytosine of the 5'-CCCGGG-3' recognition sequence. The predicted amino acid sequence of the intact Xvell endonuclease shared 41.9% identity with SmaI. However, a premature TAA translation termination codon was found in the open reading frame of xveIIR and expected to encode an 18.3 kDa truncated protein. The sequence data are consistent with observation of this study that no SmaI-like restriction activity could be detected in the cell extract of Xcv7-1.
URI: http://hdl.handle.net/11455/60370
ISSN: 0378-1097
DOI: 10.1111/j.1574-6968.2007.00738.x
Appears in Collections:分子生物學研究所

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