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標題: Immunogenicity of the envelope GP3 protein of porcine reproductive and respiratory syndrome virus displayed on baculovirus
作者: Wang, Z.S.
Xu, X.G.
Liu, H.J.
Li, Z.C.
Ding, L.
Yu, G.S.
Tong, D.W.
關鍵字: baculovirus;GP3 protein;porcine reproductive and respiratory syndrome;virus;recombinant pseudorabies virus;immune-response;prrs virus;mammalian-cells;surface display;expressing gp5;mouse model;pigs;glycoprotein;infection
Project: Acta Virologica
期刊/報告no:: Acta Virologica, Volume 55, Issue 2, Page(s) 139-146.
Porcine reproductive and respiratory syndrome virus (PRRSV) has been recognized as one of the most important pathogens of pigs throughout the world. The minor envelope protein GP3 of PRRSV plays an important role in clearing of the virus infection and protecting the animals. In this study, a recombinant baculovirus (BacSC-GP3) expressing His6-tagged GP3 with the transmembrane (TM) and cytoplasmic (CT) domains of envelope protein gp64 was constructed and its immunogenicity was evaluated in mouse and piglet models. The His6-tagged GP3 was successfully displayed on the surface of virions as well as virus-infected Sf-9 cells. The animals immunized with BacSC-GP3 gave a slightly higher (piglets) up to a markedly higher (mice) humoral and lymphocyte proliferation responses than those that received a commercial killed vaccine. This is the first study on the immunogenicity of recombinant GP3-baculovirus, which indicates that the latter can represent an alternative strategy for developing a more effective PRRSV vaccine.
ISSN: 0001-723X
DOI: 10.4149/av_2011_02_139
Appears in Collections:分子生物學研究所

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