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標題: Avian reovirus S1133-induced DNA damage signaling and subsequent apoptosis in cultured cells and in chickens
作者: Lin, P.Y.
Liu, H.J.
Chang, C.D.
Chang, C.I.
Hsu, J.L.
Liao, M.H.
Lee, J.W.
Shih, W.L.
關鍵字: hepatitis-c virus;methacrylate-induced apoptosis;acetyl-l-cysteine;oxidative stress;gene-expression;dependent apoptosis;lipid-peroxidation;protein;induction;infection
Project: Archives of Virology
期刊/報告no:: Archives of Virology, Volume 156, Issue 11, Page(s) 1917-1929.
In this study, intracellular signaling in ARV S1133-mediated apoptosis was investigated. A microarray was used to examine the gene expression profiles of cells upon ARV S1133 infection and ARV-encoded pro-apoptotic protein sigma C overexpression. The analysis indicated that in the set of DNA-damage-responsive genes, DDIT-3 and GADD45 alpha were both upregulated by viral infection and sigma C overexpression. Further investigation demonstrated that both treatments caused DNA breaks, which increased the expression and/or phosphorylation of DNA damage response proteins. ROS and lipid peroxidation levels were increased, and ARV S1133 and sigma C caused apoptosis mediated by DNA damage signaling. ROS scavenger NAC, caffeine and an ATM-specific inhibitor significantly reduced ARV S1133- and sigma C-induced DNA breaks, DDIT-3 and GADD45 alpha expression, H2AX phosphorylation, and apoptosis. Overexpression of DDIT-3 and GADD45 alpha enhanced the oxidative stress and apoptosis induced by ARV S1133 and sigma C. In conclusion, our results demonstrate the involvement of the DNA-damage-signaling pathway in ARV S1133- and sigma C-induced apoptosis.
ISSN: 0304-8608
DOI: 10.1007/s00705-011-1063-3
Appears in Collections:分子生物學研究所

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