Please use this identifier to cite or link to this item:
|標題:||Secreted expression of the VP2 protein of very virulent infectious bursal disease virus in the methylotrophic yeast Pichia pastoris||作者:||Wu, P.C.
|關鍵字:||methylotrophic yeast Pichia pastoris;very virulent infectious bursal;disease virus (vvIBDV);Mut(+) phenotype;double-stranded-rna;escherichia-coli;genomic segment;2 serotypes;sequence;strains;protection;identification;vaccination;antibodies||Project:||Journal of Virological Methods||期刊/報告no：:||Journal of Virological Methods, Volume 123, Issue 2, Page(s) 221-225.||摘要:||
The VP2-encoding gene of very virulent infectious bursa] disease virus (vvIBDV) was amplified using reverse transcription (RT)-polymerase chain reaction (PCR) and inserted into pPICZalphaA vector. Recombinant plasmid DNA was integrated into the chromosome of the transformed Pichia pastoris by electroporation and expressed protein identified by SDS-PAGE and Western blotting. High-level secreted expression was performed by determining the Mut(+) phenotype and secreting multi-copy integrants in the recombinant yeast. A recombinant protein of approximately 67 kDa was secreted into the supernatant from the yeast when induced with methanol. The expressed supernatant was bound with chicken anti-IBDV polyclonal antibodies. Western blotting with antibodies against vvIBDV indicated that the recombinant VP2 protein retained its antigenicity. High-level production (10mg/100m]) of the recombinant VP2 protein indicated that P pastoris was an efficent expression system for vvIBDV VP2 protein. (C) 2004 Elsevier B.V. All rights reserved.
|Appears in Collections:||分子生物學研究所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.