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|標題:||Molecular evolution of avian reovirus: evidence for genetic diversity and reassortment of the S-class genome segments and multiple cocirculating lineages||作者:||Liu, H.J.
|關鍵字:||cell-attachment protein;double-stranded-rna;influenza-b virus;monoclonal-antibodies;infected-cells;strain s1133;nested pcr;sigma-a;identification;nucleotide||Project:||Virology||期刊/報告no：:||Virology, Volume 314, Issue 1, Page(s) 336-349.||摘要:||
Nucleotide sequences of the S-class genome segments of 17 field-isolates and vaccine strains of avian reovirus (ARV) isolated over a 23-year period from different hosts, pathotypes, and geographic locations were examined and analyzed to define phylogenetic profiles and evolutionary mechanism. The S1 genome segment showed noticeably higher divergence than the other S-class genes. The sigmaC-encoding gene has evolved into six distinct lineages. In contrast, the other S-class genes showed less divergence than that of the sigmaC-encoding gene and have evolved into two to three major distinct lineages, respectively. Comparative sequence analysis provided evidence indicating extensive sequence divergence between ARV and other orthoreoviruses. The evolutionary trees of each gene were distinct, suggesting that these genes evolve in an independent manner. Furthermore, variable topologies were the result of frequent genetic reassortment among multiple cocirculating lineages. Results showed genetic diversity correlated more closely with date of isolation and geographic sites than with host species and pathotypes. This is the first evidence demonstrating genetic variability among circulating ARVs through a combination of evolutionary mechanisms involving multiple cocirculating lineages and genetic reassortment. The evolutionary rates and patterns of base substitutions were examined. The evolutionary rate for the sigmaC-encoding gene and sigmaC protein was higher than for the other S-class genes and other family of viruses. With the exception of the sigmaC-encoding gene, which nonsynonymous substitutions predominate over synonymous, the evolutionary process of the other S-class genes can be explained by the neutral theory of molecular evolution. Results revealed that synonymous substitutions predominate over nonsynonymous in the S-class genes, even though genetic diversity and substitution rates vary among the viruses. (C) 2003 Elsevier Inc. All rights reserved.
|Appears in Collections:||分子生物學研究所|
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